Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, India.
Bioprocess Biosyst Eng. 2012 Sep;35(7):1147-56. doi: 10.1007/s00449-012-0700-x. Epub 2012 Feb 14.
1,3-propanediol (1,3-PD) is a chemical compound of immense importance primarily used as a raw material for fiber and textile industry. It can be produced by the fermentation of glycerol available abundantly as a by-product from the biodiesel plant. The present study was aimed at determination of key kinetic parameters of 1,3-PD fermentation by Clostridium diolis. Initial experiments on microbial growth inhibition were followed by optimization of nutrient medium recipe by statistical means. Batch kinetic data from studies in bioreactor using optimum concentration of variables obtained from statistical medium design was used for estimation of kinetic parameters of 1,3-PD production. Direct use of raw glycerol from biodiesel plant without any pre-treatment for 1,3-PD production using this strain investigated for the first time in this work gave results comparable to commercial glycerol. The parameter values obtained in this study would be used to develop a mathematical model for 1,3-PD to be used as a guide for designing various reactor operating strategies for further improving 1,3-PD production. An outline of protocol for model development has been discussed in the present work.
1,3-丙二醇（1,3-PD）是一种非常重要的化学化合物，主要用作纤维和纺织工业的原料。它可以通过发酵生物柴油工厂大量产生的副产品甘油来生产。本研究旨在确定 Clostridium diolis 发酵 1,3-PD 的关键动力学参数。首先进行微生物生长抑制的初步实验，然后通过统计方法对营养培养基配方进行优化。使用从统计培养基设计中获得的最佳变量浓度在生物反应器中进行分批动力学数据研究，用于估计 1,3-PD 生产的动力学参数。本工作首次研究了使用该菌株直接使用生物柴油厂的原始甘油进行 1,3-PD 生产，无需任何预处理，结果可与商业甘油相媲美。本研究中获得的参数值将用于开发 1,3-PD 的数学模型，作为设计各种反应器操作策略的指南，以进一步提高 1,3-PD 的产量。本工作讨论了模型开发的方案大纲。
