Center for Environmental Technology Research, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul 136-791, South Korea.
Appl Biochem Biotechnol. 2010 May;161(1-8):502-10. doi: 10.1007/s12010-009-8859-6. Epub 2009 Nov 25.
The microbial production of 1,3-propanediol (1,3-PD) from raw glycerol, a byproduct of biodiesel production, is economically and environmentally advantageous. Although direct use of raw glycerol without any pretreatment is desirable, previous studies have reported that this could cause inhibition of microbial growth. In this study, we investigated the effects of raw glycerol type, different microorganisms, and pretreatment of raw glycerol on the production of 1,3-PD. Raw glycerol from waste vegetable-oil-based biodiesel production generally caused more inhibition of 1,3-PD production and microbial growth compared to raw glycerol from soybean-oil-based biodiesel production. In addition, two raw glycerol types produced from two biodiesel manufacturers using waste vegetable oil exhibited different 1,3-PD production behavior, partially due to different amounts of methanol included in the raw glycerol from the two biodiesel manufacturers. Klebsiella strains were generally resistant to all types of raw glycerol while the growth of Clostridium strains was variably inhibited depending on the type of raw glycerol. The 1,3-PD production of the Clostridium strains using acid-pretreated raw glycerol was significantly enhanced compared to that with raw glycerol, demonstrating the feasibility of using raw glycerol for 1,3-PD production by various microorganisms.
利用生物柴油生产的副产物粗甘油来微生物生产 1,3-丙二醇（1,3-PD）在经济和环境方面具有优势。虽然直接使用未经任何预处理的粗甘油是理想的，但之前的研究表明，这可能会抑制微生物的生长。在本研究中，我们研究了粗甘油的类型、不同微生物以及粗甘油的预处理对 1,3-PD 生产的影响。与来自大豆油基生物柴油生产的粗甘油相比，来自废植物油基生物柴油生产的粗甘油通常会对 1,3-PD 生产和微生物生长产生更大的抑制作用。此外，来自两家使用废植物油生产生物柴油的制造商的两种粗甘油类型表现出不同的 1,3-PD 生产行为，部分原因是来自这两家生物柴油制造商的粗甘油中包含的甲醇量不同。克雷伯氏菌菌株通常对所有类型的粗甘油都有抗性，而梭菌菌株的生长则根据粗甘油的类型而不同程度地受到抑制。与使用粗甘油相比，用酸预处理的粗甘油培养的梭菌菌株的 1,3-PD 生产显著增强，这表明各种微生物利用粗甘油生产 1,3-PD 是可行的。
