Department of Chemistry, Amirkabir University of Technology, Tehran, Iran.
J Mater Sci Mater Med. 2012 Apr;23(4):963-72. doi: 10.1007/s10856-012-4565-y. Epub 2012 Feb 14.
Water-compatible imprinted nanoparticles were prepared for carbamazepine as a template and used for the selective extraction and controlled release of carbamazepine. Assay materials and drug delivery carriers were typically used in aqueous environments, so it is generally preferable to prepare solvent-free molecularly imprinted nanoparticles in water using the miniemulsion polymerization method. The present work investigates a bio-analytical strategy generically applicable to imprinted materials for molecular recognition studies, including equilibrium and non-equilibrium binding, and release experiments, increasing the knowledge of the molecular interactions between the template molecules and imprinted nanoparticles. The results showed that the imprinted nanoparticles exhibited a higher binding level and slower release rate than non-imprinted nanoparticles. The selectivity of imprinted nanoparticles for carbamazepine studied in comparison with an analogue compound, oxcarbazepine, the main metabolite of carbamazepine. The recovery and selectivity of carbamazepine in human serum was determined to be 100%, 1.7 times that of oxcarbazepine. The results indicated that carbamazepine-imprinted nanoparticles are appropriate for serum level determination of the drug in therapeutic range. The template to functional monomer ratio as a key factor controlling the recognition and release kinetic mechanism of imprinted nanoparticles is discussed. The imprinted nanoparticles prepared at the appropriate template to functional monomer mole ratio (2:8) exhibited the best drug affinity (5.1 times higher) and a slower drug release rate due to the interaction of carbamazepine with the imprinted cavities within the nanoparticles. Loaded imprinted nanoparticles as drug reservoirs were able to prolong carbamazepine release, in 1% wt sodium dodecyl sulfate aqueous solution, for more than 8 days.
水相兼容的印迹纳米粒子被制备用于卡马西平作为模板，并用于卡马西平的选择性提取和控制释放。分析材料和药物递送载体通常在水相环境中使用，因此通常优选使用 miniemulsion 聚合方法在水中制备无溶剂的分子印迹纳米粒子。本工作研究了一种普遍适用于分子识别研究的印迹材料的生物分析策略，包括平衡和非平衡结合以及释放实验，增加了模板分子与印迹纳米粒子之间分子相互作用的知识。结果表明，印迹纳米粒子表现出比非印迹纳米粒子更高的结合水平和更慢的释放速率。与类似物化合物奥卡西平相比，印迹纳米粒子对卡马西平的选择性研究，奥卡西平是卡马西平的主要代谢物。在人血清中测定卡马西平的回收率和选择性为 100%，是奥卡西平的 1.7 倍。结果表明，卡马西平印迹纳米粒子适用于治疗范围内药物血清水平的测定。讨论了作为控制印迹纳米粒子识别和释放动力学机制的关键因素的模板与功能单体的比例。在适当的模板与功能单体摩尔比（2：8）下制备的印迹纳米粒子表现出最佳的药物亲和力（高 5.1 倍）和较慢的药物释放速率，这是由于卡马西平与纳米粒子内的印迹腔相互作用的结果。作为药物储库的负载印迹纳米粒子能够在 1%wt 十二烷基硫酸钠水溶液中延长卡马西平的释放时间，超过 8 天。
