Belmont Anne-Sophie, Jaeger Stefanie, Knopp Dietmar, Niessner Reinhard, Gauglitz Guenter, Haupt Karsten
Lund University, Pure and Applied Biochemistry, Sweden.
Biosens Bioelectron. 2007 Jun 15;22(12):3267-72. doi: 10.1016/j.bios.2007.01.023. Epub 2007 Feb 11.
Reflectometric interference spectroscopic measurements were performed on molecularly imprinted polymer (MIP) films with the herbicide atrazine as the template molecule. A conventional imprinting protocol was used relying on non-covalent interactions between the functional monomers and the template. The MIPs were deposited on glass transducers by two different methods: spin-coating followed by in situ polymerization of thin films of monomers containing a sacrificial polymeric porogen, and autoassembly of MIP nanoparticles with the aid of an associative linear polymer. Reproducible results were obtained upon measurements of atrazine solutions in toluene with both films. Atrazine concentrations as low as 1.7 ppm could be detected with the autoassembled particle film. No or very little analyte adsorption was observed onto non-imprinted control films made by spin-coating and by particle assembly, respectively. We believe that these MIP layers in combination with the general reflectrometric transduction scheme could be a versatile sensing tool for the detection of environmentally important and other analytes.
以除草剂阿特拉津为模板分子，对分子印迹聚合物（MIP）薄膜进行反射干涉光谱测量。采用了依赖功能单体与模板之间非共价相互作用的传统印迹方案。通过两种不同方法将MIP沉积在玻璃换能器上：旋涂后原位聚合含牺牲性聚合物致孔剂的单体薄膜，以及借助缔合线性聚合物自组装MIP纳米颗粒。用这两种薄膜测量甲苯中的阿特拉津溶液时均获得了可重复的结果。使用自组装颗粒薄膜可检测低至1.7 ppm的阿特拉津浓度。分别通过旋涂和颗粒组装制备的非印迹对照薄膜上未观察到或仅观察到极少的分析物吸附。我们认为，这些MIP层与一般的反射传感方案相结合，可能成为检测对环境重要的分析物及其他分析物的通用传感工具。
