Department of Pediatric Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
J Mater Sci Mater Med. 2012 Apr;23(4):1109-17. doi: 10.1007/s10856-012-4576-8. Epub 2012 Feb 14.
A major challenge facing the development of tissue-engineered vascular grafts (TEVGs), promising living replacements for diseased vascular structures, is enhancing angiogenesis. To promote rapid vascularization, endothelial cells (ECs) were co-cultured with smooth muscle cells (SMCs) in decellularized small intestinal submucosa scaffolds to regenerate angiogenic-TEVGs (A-TEVGs). Observation of the A-TEVGs at 1 month post-implantation revealed that a rich network of neocapillaries lining the blood vessel wall had developed; that the ECs of the neovasculatures had been derived from previously seeded ECs and later invading ECs of the host's vascular bed; that tissue vascularization had not significantly impaired mechanical properties; and that the maximal tensile strength of the A-TEVGs was of the same order of magnitude as that of native porcine femoral arteries. These results indicate that of the co-culturing of ECs with SMCs could enhance vascularization of TEVGs in vivo, possibly increasing graft perfusion and host integration.
组织工程血管移植物（TEVGs）的发展面临着一个主要挑战，即增强血管生成，以提供有希望的患病血管结构的活体替代品。为了促进快速血管化，将内皮细胞（ECs）与平滑肌细胞（SMCs）共培养在脱细胞的小肠黏膜下层支架中，以再生血管生成 TEVGs（A-TEVGs）。在植入后 1 个月观察 A-TEVGs 时发现，血管壁上已经形成了丰富的新毛细血管网络；新生血管的 ECs 来源于先前接种的 ECs 和后来入侵宿主血管床的 ECs；组织血管化并没有显著损害机械性能；A-TEVGs 的最大拉伸强度与天然猪股动脉处于同一数量级。这些结果表明，ECs 与 SMCs 的共培养可以增强体内 TEVGs 的血管生成，可能增加移植物灌注和宿主整合。
