Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
J Mater Sci Mater Med. 2012 Apr;23(4):931-41. doi: 10.1007/s10856-012-4577-7. Epub 2012 Feb 14.
Electrospun gelatin and poly-ε-caprolactone (PCL) nanofibers were prepared using needleless technology and their biocompatibility and therapeutic efficacy have been characterized in vitro in cell cultures and in an experimental model of a skin wound. Human dermal fibroblasts, keratinocytes and mesenchymal stem cells seeded on the nanofibers revealed that both nanofibers promoted cell adhesion and proliferation. The effect of nanofibers on wound healing was examined using a full thickness wound model in rats and compared with a standard control treatment with gauze. Significantly faster wound closure was found with gelatin after 5 and 10 days of treatment, but no enhancement with PCL nanofibers was observed. Histological analysis revealed enhanced epithelialisation, increased depth of granulation tissue and increased density of myofibroblasts in the wound area with gelatin nanofibers. The results show that gelatin nanofibers produced by needleless technology accelerate wound healing and may be suitable as a scaffold for cell transfer and skin regeneration.
静电纺丝明胶和聚己内酯（PCL）纳米纤维采用无针技术制备，其体外细胞培养和皮肤创面实验模型中的生物相容性和治疗效果已得到表征。在纳米纤维上接种的人真皮成纤维细胞、角质形成细胞和间充质干细胞表明，两种纳米纤维都促进了细胞的黏附和增殖。通过大鼠全层创面模型，将纳米纤维的作用与纱布的标准对照治疗进行了比较。结果发现，治疗 5 天和 10 天后，明胶的创面闭合速度明显更快，但 PCL 纳米纤维没有增强作用。组织学分析显示，明胶纳米纤维促进了上皮化，增加了肉芽组织的深度，并增加了创面处肌成纤维细胞的密度。结果表明，无针技术制备的明胶纳米纤维可加速创面愈合，可作为细胞转移和皮肤再生的支架。
