Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
Int J Biol Macromol. 2019 Mar 1;124:478-491. doi: 10.1016/j.ijbiomac.2018.11.237. Epub 2018 Nov 27.
In this study, we aimed to evaluate the role of electrospun nanofibers containing lawsone (2-hydroxy-1,4-naphthoquinone) in wound healing. Different concentrations of lawsone (0.5%, 1%, 1.5%) were electrospun in polycaprolactone-gelatin (PCL/Gel) polymers in core-shell architecture. Nanofibers were characterized using scanning electron microscopy (SEM), Transmission electron microscopy (TEM). Coaxial electrospinning of PCL/Gel/lawsone (PCL/Gel/Law) scaffolds prolonged the release of lawsone over a period of 20 days. In vitro bioactivity of fibers on human gingiva fibroblast cells (HGF) was evaluated by MTT assay. The PCL/Gel/Law 1% increased cell attachment and proliferation significantly. Additionally, the in vitro gene expression of transforming growth factor β (TGF-B1), collagen (COL1) and epidermal growth factor (EGF) was monitored using RT-qPCR technique, which there was significant increase in TGF-B1 and COL1 gene expression in PCL/Gel/Law 0.5% and 1% mats. In vivo wound healing activity of lawsone loaded scaffolds in rat wound model revealed that the PCL/Gel/Law 1% had the highest impact on healing by increasing re-epithelialization of the wound after 14 days. It can be inferred that lawsone 1% incorporation in the core of PCL/Gel electrospun nanofibers has excellent characteristics and can be used as wound dressing patch in medicine.
在这项研究中，我们旨在评估含有 lawsone（2-羟基-1,4-萘醌）的电纺纳米纤维在伤口愈合中的作用。不同浓度的 lawsone（0.5%、1%、1.5%）以核壳结构形式被电纺到聚己内酯-明胶（PCL/Gel）聚合物中。纳米纤维通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）进行了表征。PCL/Gel/lawsone（PCL/Gel/Law）支架的同轴电纺延长了 lawsone 在 20 天内的释放。通过 MTT 测定法评估纤维对人牙龈成纤维细胞（HGF）的体外生物活性。PCL/Gel/Law 1%显著增加了细胞黏附和增殖。此外，使用 RT-qPCR 技术监测了转化生长因子β（TGF-B1）、胶原蛋白（COL1）和表皮生长因子（EGF）的体外基因表达，发现 PCL/Gel/Law 0.5%和 1%垫中 TGF-B1 和 COL1 基因表达显著增加。 lawsone 负载支架在大鼠伤口模型中的体内伤口愈合活性表明，PCL/Gel/Law 1% 通过增加 14 天后伤口的再上皮化，对愈合有最大的影响。可以推断，PCL/Gel 电纺纳米纤维中 lawsone 1%的掺入具有优异的特性，可作为医学上的伤口敷料贴片使用。
