UBC James Hogg Research Centre, Institute for Heart + Lung Health at St, Paul's Hospital and Department of Medicine, University of British Columbia, Vancouver, BC, Canada.
BMC Pulm Med. 2012 Feb 14;12:3. doi: 10.1186/1471-2466-12-3.
In cystic fibrosis (CF) patients, it has been suggested that systemic inflammation may be an important risk factor for poor health outcomes. The relationship of plasma inflammatory biomarkers to lung function and hospitalization history remains largely unexplored.
This cross-sectional study included 58 consecutive, clinically stable adults from the CF Clinic at St. Paul's Hospital (Vancouver, Canada). Blood levels of interleukin (IL)-6, IL-1β, C-reactive protein (CRP), interleukin (IL)-6, IL-1β, granzyme B (GzmB), chemokine C-C motif ligand 18 (CCL18/PARC), surfactant protein D (SP-D), lipopolysaccharide (LPS)-binding protein, and soluble cluster of differentiation 14 (sCD14) were measured using enzyme-linked immunosorbent assays, and LPS levels were measured using a Limulus amebocyte lysate assay. Spirometry was also performed. Multivariable linear regression analysis was used to assess relationships of the blood biomarkers to lung function.
Lung function impairment was independently associated with elevated plasma levels of CRP (P < 0.01), IL-6 (P = 0.04), IL-1β (P < 0.01), and LBP (P < 0.01). Increasing age (P < 0.01), reduced body mass index (P = 0.02), prior hospitalizations (P = 0.03), and presence of Pseudomonas aeruginosa in sputum cultures (P < 0.01) were also associated with reduced lung function. Elevated concentrations of LPS in plasma were associated with a previous history of hospitalization (P < 0.05). There was a trend towards an increase in plasma IL-6 (P = 0.07) and IL-1β (P = 0.06) levels in patients who were previously hospitalized.
IL-6 and IL-1β are promising systemic biomarkers for lung function impairment and history of hospitalization in adult patients with CF.
在囊性纤维化（CF）患者中，全身性炎症可能是健康状况不佳的重要危险因素，这一观点已得到提出。然而，血浆炎症生物标志物与肺功能和住院史之间的关系在很大程度上仍未得到探索。
本横断面研究纳入了来自加拿大温哥华圣保罗医院 CF 诊所的 58 例连续、临床稳定的成年患者。采用酶联免疫吸附试验检测白细胞介素（IL）-6、IL-1β、C 反应蛋白（CRP）、IL-6、IL-1β、颗粒酶 B（GzmB）、趋化因子 C-C 基序配体 18（CCL18/PARC）、表面活性剂蛋白 D（SP-D）、脂多糖（LPS）结合蛋白和可溶性 CD14 的水平，并采用鲎变形细胞溶解物测定法检测 LPS 水平。同时进行了肺功能检查。采用多元线性回归分析评估血液生物标志物与肺功能之间的关系。
肺功能障碍与 CRP（P < 0.01）、IL-6（P = 0.04）、IL-1β（P < 0.01）和 LBP（P < 0.01）血浆水平升高独立相关。年龄增加（P < 0.01）、体重指数降低（P = 0.02）、既往住院（P = 0.03）以及痰液培养中铜绿假单胞菌的存在（P < 0.01）也与肺功能下降相关。血浆中 LPS 浓度升高与既往住院史相关（P < 0.05）。既往住院的患者，血浆中 IL-6（P = 0.07）和 IL-1β（P = 0.06）水平呈升高趋势。
IL-6 和 IL-1β 是 CF 成年患者肺功能障碍和住院史的有前途的系统性生物标志物。
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