Department of Chemistry, Jadavpur University, Kolkata 700 032, India.
Org Biomol Chem. 2012 Mar 28;10(12):2380-4. doi: 10.1039/c2ob07084g. Epub 2012 Feb 15.
A highly selective and sensitive fluorescent Zn(2+) sensor, 2,6-bis(2-hydroxy-benzoic acid hydrazide)-4-methylphenol (1), was designed and synthesized. In aqueous THF (4 : 6 v/v) ligand 1 induces a 2 : 1 complex formation with respect to Zn(2+) at physiological pH. This probe features visible light excitation(390 nm) and emission (490 nm) profiles, excellent selectivity responses for Zn(2+)over other competing biological metal ions with K(d) < 1 pM(2), LOD < 1 ng L(-1) and about 680 fold enhancement in fluorescent intensity upon Zn(2+) binding. It also exhibits cell permeability and intracellular Zn(2+) sensing in A375 human melanoma cancer cell.
一种高选择性和高灵敏度的荧光锌（Ⅱ）传感器，2,6-双（2-羟基苯甲酰肼基）-4-甲基苯酚（1），被设计和合成。在水与四氢呋喃（4:6 v/v）的混合溶液中，配体 1 在生理 pH 值下与锌（Ⅱ）以 2:1 的比例形成配合物。该探针具有可见光激发（390nm）和发射（490nm）谱，对锌（Ⅱ）具有优异的选择性响应，对其他竞争的生物金属离子的检测限低于 1 pM（2），锌（Ⅱ）结合后的荧光强度增强约 680 倍。它还具有细胞通透性，并能在 A375 人黑色素瘤癌细胞内检测锌（Ⅱ）。
