Department of Food Hygiene and Technology, Uludag University, Gorukle Campus, 16059, Bursa, Turkey.
Poult Sci. 2012 Mar;91(3):724-31. doi: 10.3382/ps.2011-01863.
This study was conducted to evaluate the capability of the Vitek immunodiagnostic assay system easy Salmonella (VIDAS ESLM) method and a specific real-time PCR system (LightCycler, LCPCR) to complement the International Organization for Standardization Method 6579 (ISO) in detecting Salmonella from a total of 105 naturally contaminated samples comprised of poultry meat and poultry meat products. The detection limit of ISO and LCPCR was 9 cfu/mL for both poultry meat and poultry meat products, whereas that of VIDAS ESLM with both sample types was determined to be 90 cfu/mL. Twelve (33.33%), 11 (30.55%), and 18 (50.00%) out of 36 poultry meat samples were positive for Salmonella by ISO, VIDAS ESLM, and LCPCR, respectively. Salmonella detection rates from poultry meat products were 5.80% for ISO and 8.69% for LCPCR, whereas none of these products tested positive by VIDAS ESLM. In poultry meat samples, VIDAS ESLM and LCPCR detection results were in substantial agreement with ISO, with the relative accuracy, sensitivity, and specificity rates of 97.2, 91.7, and 100%, respectively, for VIDAS ESLM and 83.3, 100, and 75%, respectively, for LCPCR. This is the first report on the evaluation of both VIDAS ESLM and LCPCR to complement ISO for the rapid detection of Salmonella in poultry meat and meat products. We determined that both VIDAS ESLM and LCPCR have the potential to complement the ISO standard culture method in the rapid screening of Salmonella from naturally contaminated poultry meats. For the poultry meat products, VIDAS ESLM and LCPCR can be used for rapid primary screening, and they should be complemented absolutely by ISO. Although LCPCR can preferentially be used for initial screening poultry meat products, the results should definitely be confirmed by ISO. Also, the VIDAS ESLM did not seem to be a suitable method for detecting Salmonella in poultry meat products.
本研究旨在评估 Vitek 免疫诊断检测系统简便沙门氏菌（VIDAS ESLM）方法和特定实时 PCR 系统（LightCycler，LCPCR）在检测来自共 105 份天然污染样本（包括禽肉和禽肉产品）中沙门氏菌方面对国际标准化组织方法 6579（ISO）的补充能力。ISO 和 LCPCR 对禽肉和禽肉产品的检测限均为 9 cfu/mL，而 VIDAS ESLM 对两种样本类型的检测限则分别确定为 90 cfu/mL。ISO、VIDAS ESLM 和 LCPCR 检测到 36 份禽肉样本中分别有 12 份（33.33%）、11 份（30.55%）和 18 份（50.00%）为沙门氏菌阳性。ISO 检测到禽肉产品中的沙门氏菌检出率为 5.80%，LCPCR 为 8.69%，而 VIDAS ESLM 检测结果均为阴性。在禽肉样本中，VIDAS ESLM 和 LCPCR 的检测结果与 ISO 基本一致，VIDAS ESLM 的相对准确性、灵敏度和特异性分别为 97.2%、91.7%和 100%，LCPCR 分别为 83.3%、100%和 75%。这是首次评估 VIDAS ESLM 和 LCPCR 对 ISO 用于快速检测禽肉和禽肉产品中沙门氏菌的补充作用。我们确定 VIDAS ESLM 和 LCPCR 均有潜力在快速筛选天然污染禽肉中的沙门氏菌方面补充 ISO 标准培养方法。对于禽肉产品，VIDAS ESLM 和 LCPCR 可用于快速初步筛选，且必须绝对补充 ISO。虽然 LCPCR 可优先用于初步筛选禽肉产品，但结果绝对需要通过 ISO 确认。此外，VIDAS ESLM 似乎不适合检测禽肉产品中的沙门氏菌。
