Pun R, Pant K P, Bhatta D R, Pandey B D
Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal.
JNMA J Nepal Med Assoc. 2011 Jan-Mar;51(181):11-4.
Dengue fever is an emerging mosquito borne disease in Nepal claiming substantial morbidity and mortality. The objective of the study was to find out frequency of acute dengue infection in patients from the hospitals of the western Nepal.
The study was conducted between August 2007 and July 2008 in patients visiting hospitals of the western terai of Nepal with chief complains of fever. The sero-diagnosis of acute dengue infection was determined by enzyme linked immunosorbent assay among 239 patients visiting Lumbini Zonal Hospital, Butwal; Bheri Zonal Hospital, Nepalgunj; Bardiya District Hospital, Bardiya and Mahakali Zonal Hospital, Mahendranagar.
The anti-dengue IgM positivity was 29.3%. There was slight male preponderance with a male to female ratio of 1.2:1. Out of the total positive cases, the highest positive cases (75.7%) were from the age group 15 - 50 years followed by < 15 years old (15.7%). Out of four hospitals, the highest positive cases (54.3%) were in Lumbini Zonal Hospital, Butwal. The age and gender were independent predictors to dengue virus infection. The highest numbers of dengue positive cases were in October (52.6%). The association between dengue disease and the month was statistically significant.
The dengue positivity was estimated in acute patients from hospitals of western Nepal by enzyme immunoassay. Therefore, the serological marker can be used to diagnose acute patients of dengue during outbreaks.
登革热是尼泊尔一种新出现的蚊媒疾病，导致大量发病和死亡。本研究的目的是查明尼泊尔西部医院患者中急性登革热感染的频率。
本研究于2007年8月至2008年7月在尼泊尔西部特莱地区医院就诊、主诉为发热的患者中进行。在到访蓝毗尼地区医院（布特瓦尔）、贝里地区医院（尼泊尔根杰）、巴迪亚地区医院（巴迪亚）和马哈卡利地区医院（马亨德拉纳加尔）的239名患者中，通过酶联免疫吸附测定法对急性登革热感染进行血清学诊断。
抗登革热IgM阳性率为29.3%。男性略占优势，男女比例为1.2:1。在所有阳性病例中，最高阳性病例数（75.7%）来自15 - 50岁年龄组，其次是<15岁年龄组（15.7%）。在四家医院中，最高阳性病例数（54.3%）出现在蓝毗尼地区医院（布特瓦尔）。年龄和性别是登革热病毒感染的独立预测因素。登革热阳性病例数最多的月份是10月（52.6%）。登革热疾病与月份之间的关联具有统计学意义。
通过酶免疫测定法对尼泊尔西部医院急性患者的登革热阳性情况进行了评估。因此，血清学标志物可用于在疫情暴发期间诊断急性登革热患者。
