[高氯酸铵对雄性大鼠甲状腺功能和睾丸的影响]

[The effects of ammonium perchlorate on thyroid function and testis in male rats].

作者信息

Zhang Rao, Wu Feng-Hong, Zhou Xuan, Peng Kai-Liang

机构信息

Public Health School, Tongli Medical College, Huazhong University of Science and Technology, Wuhan, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2011 Aug;29(8):579-82.

PMID:22335154

Abstract

OBJECTIVE

To study the effects of ammonium perchlorate (AP) on the levels of thyroid hormone and the testis function of male rats.

METHODS

Twenty male rats were randomly divided into 4 groups: control group, low, middle and high AP group. The rats were exposed orally to 0, 130, 260 and 520 mg AP/kg a day for 80 days. The levels of thyroid hormone, testosterone in serum and sperm motility were measured and the testis histological change was observed as well.

RESULTS

The increase of body weight in high AP group was significantly lower than that in the control group (P < 0.01). The organ coefficients of testis and thyroid in high AP group obviously enhanced, as compared with the control group (P < 0.01). The free thyroxin (FT4) levels of serum in all AP treated groups were significantly lower than that of the control group (P < 0.05). There were no differences of serum FT3 levels between all AP groups and control group, while serum TSH levels in middle and high AP groups decreased significantly, as compared with control group (P < 0.01). In terms of sperm motility, the percentage of Grade A and B sperm in middle and high groups were 12.3% +/- 2.52% and 14.8% +/- 5.93%, 17.7% +/- 4.63%, 15.8% +/- 2.28% respectively, which were significantly lower than that (27.8% +/- 8.70%) in control group (P < 0.01). The percentage of Grade D sperm in middle and high groups were 38.0% +/- 3.61% and 40.0% +/- 8.99%, respectively, which were significantly higher than that (17.0% +/- 5.00%) in control group (P < 0.01). No difference of serum testosterone level between all AP groups and control group was observed.

CONCLUSION

AP can influence the levels of thyroid hormone and reduce the serum FT4 levels in rats. The main toxic effects on male reproductive system may decrease the sperm motility.

摘要

目的

研究高氯酸铵（AP）对雄性大鼠甲状腺激素水平及睾丸功能的影响。

方法

将20只雄性大鼠随机分为4组：对照组、低、中、高AP组。大鼠每天经口给予0、130、260和520 mg AP/kg，持续80天。检测血清甲状腺激素、睾酮水平及精子活力，并观察睾丸组织学变化。

结果

高AP组体重增加明显低于对照组（P < 0.01）。高AP组睾丸和甲状腺的脏器系数明显高于对照组（P < 0.01）。各AP处理组血清游离甲状腺素（FT4）水平均显著低于对照组（P < 0.05）。各AP组与对照组血清FT3水平无差异，而中、高AP组血清促甲状腺激素（TSH）水平显著低于对照组（P < 0.01）。在精子活力方面，中、高剂量组A级和B级精子百分比分别为12.3%±2.52%、14.8%±5.93%，17.7%±4.63%、15.8%±2.28%，均显著低于对照组（27.8%±8.70%）（P < 0.01）。中、高剂量组D级精子百分比分别为38.0%±3.61%、40.0%±8.99%，显著高于对照组（17.0%±5.00%）（P < 0.01）。各AP组与对照组血清睾酮水平无差异。