Chen Hong Xia, Ding Miao Hong, Li Yong Gan, Liu Qin, Peng Kai Liang
a Institute of Biomedicine, Taihe Hospital , Hubei University of Medicine , Shiyan , People's Republic of China.
Arch Environ Occup Health. 2015;70(5):286-90. doi: 10.1080/19338244.2014.904265.
To evaluate the feasibility of urine perchlorate as a biomarker of ammonium perchlorate (AP) exposure and to explore the correlation between the thyroid function indicators and the perchlorate concentrations, a sensitive and selective ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) method was developed to detect perchlorate in urine samples. Rats were orally administrated with different doses of perchlorate. Serum free thyroxine (FT4), free triiodothyronine (FT3), and thyroid-stimulating hormone (TSH) were determined by radioimmunoassays. The results showed that a dose of AP up to 520 mg kg(-1) body weight induced a significant increase of TSH, with a decrease of FT4. Particularly, the levels of urine perchlorate increased dose-dependently on AP exposure from drinking water. The findings highlighted that urine perchlorate may be a useful biomarker for AP environmental exposure.
为评估尿中高氯酸盐作为高氯酸铵（AP）暴露生物标志物的可行性，并探讨甲状腺功能指标与高氯酸盐浓度之间的相关性，开发了一种灵敏且选择性高的超高效液相色谱-质谱联用（UHPLC-MS）方法来检测尿样中的高氯酸盐。给大鼠口服不同剂量的高氯酸盐。通过放射免疫分析法测定血清游离甲状腺素（FT4）、游离三碘甲状腺原氨酸（FT3）和促甲状腺激素（TSH）。结果表明，高达520 mg kg(-1)体重的AP剂量可导致TSH显著升高，FT4降低。特别是，尿中高氯酸盐水平随饮用水中AP暴露剂量的增加而呈剂量依赖性升高。这些发现突出表明，尿中高氯酸盐可能是AP环境暴露的有用生物标志物。
