使用门控 13N-NH3 微 PET 检查大鼠左心室功能。

Use of gated 13N-NH3 micro-PET to examine left ventricular function in rats.

机构信息

Department of Cardiology, University Medical Center Groningen, University of Groningen, P.O. Box 30001, 9700 RB Groningen, The Netherlands.

出版信息

Nucl Med Biol. 2012 Jul;39(5):724-9. doi: 10.1016/j.nucmedbio.2011.12.009. Epub 2012 Feb 14.

DOI:10.1016/j.nucmedbio.2011.12.009

PMID:22336370

Abstract

INTRODUCTION

Myocardial perfusion gating techniques offer the possibility of measurement of left ventricular end-systolic (ESV) and end-diastolic volume (EDV) and left ventricular ejection fraction (LVEF) in clinical and preclinical trials. The aim of this study was to evaluate left ventricular volumes (LVV) and LVEF with 13N-NH3 in comparison with the reference 18F-FDG in different rat models.

METHODS

In this study, 18 male Wistar rats, 12 control rats and 6 rats with myocardial infarction (MI) were imaged with micro-PET. The ratswere scanned with gated 13N-NH3 and 18F-FDG sequentially for the assessment of LVV and LVEF. A validated three-dimensional segmentation algorithm was used to calculate LVV and LVEF.

RESULTS

Mean LVEF measured with 13N-NH3 was 45.6±8.9 and 75.3±9.4%, mean ESV was 0.40±0.12 and 0.14±0.11 ml, and mean EDVwas 0.53±16 and 0.75±0.18 ml for MI and control rats, respectively. Moderate to good correlations were observed between values of 13N-NH3 and 18F-FDG for calculation of ESV [r=0.80, P<.0001, standard error of estimate (SEE)=0.10], EDV (r=0.63, P=.005, SEE=0.14) and LVEF (r=0.84, P<.0001, SEE=9.5). LVEF measured with 13N-NH3 was significantly lower in MI rats in comparison to measurement with 18F-FDG (45.6±8.9 vs 54.9±9.3 %; P=.04).

CONCLUSION

Correlations were moderate to good for the assessment of ESV, EDV and LVEF between gated 13N-NH3 and 18F-FDG. LVEF was underestimated with gated 13N-NH3 in rats with myocardial infarction. In healthy rats, LV volumes and LVEF can be measured reproducibly with either approach.

摘要

介绍

心肌灌注门控技术提供了在临床前和临床试验中测量左心室收缩末期（ESV）和舒张末期容积（EDV）和左心室射血分数（LVEF）的可能性。本研究的目的是评估 13N-NH3 与参考 18F-FDG 在不同大鼠模型中的左心室容积（LVV）和 LVEF。

方法

在这项研究中，18 只雄性 Wistar 大鼠，12 只对照大鼠和 6 只心肌梗死（MI）大鼠接受 micro-PET 成像。大鼠依次进行门控 13N-NH3 和 18F-FDG 扫描，以评估 LVV 和 LVEF。使用经过验证的三维分割算法来计算 LVV 和 LVEF。

结果

用 13N-NH3 测量的平均 LVEF 分别为 45.6±8.9 和 75.3±9.4%，平均 ESV 分别为 0.40±0.12 和 0.14±0.11 ml，平均 EDV 分别为 0.53±16 和 0.75±0.18 ml，用于 MI 和对照大鼠。观察到 13N-NH3 值与 18F-FDG 值之间存在中度至良好的相关性，用于计算 ESV[r=0.80，P<.0001，标准误差估计（SEE）=0.10]、EDV[r=0.63，P=.005，SEE=0.14]和 LVEF[r=0.84，P<.0001，SEE=9.5]。与 18F-FDG 相比，MI 大鼠中用 13N-NH3 测量的 LVEF 显著降低（45.6±8.9 与 54.9±9.3%；P=.04）。