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本文引用的文献

1

Outbreak of Shiga toxin-producing Escherichia coli (STEC) O104:H4 infection in Germany causes a paradigm shift with regard to human pathogenicity of STEC strains.德国爆发的产志贺毒素大肠杆菌（STEC）O104:H4 感染导致了人们对 STEC 菌株的致病性的观念发生了转变。

J Food Prot. 2012 Feb;75(2):408-18. doi: 10.4315/0362-028X.JFP-11-452.

2

A rapid procedure for the detection and isolation of enterohaemorrhagic Escherichia coli (EHEC) serogroup O26, O103, O111, O118, O121, O145 and O157 strains and the aggregative EHEC O104:H4 strain from ready-to-eat vegetables.一种快速检测和分离即食蔬菜中肠出血性大肠杆菌（EHEC）血清群 O26、O103、O111、O118、O121、O145 和 O157 菌株以及聚集性 EHEC O104:H4 菌株的快速程序。

Int J Food Microbiol. 2012 Jan 3;152(1-2):19-30. doi: 10.1016/j.ijfoodmicro.2011.10.009. Epub 2011 Oct 21.

3

Treatment of severe neurological deficits with IgG depletion through immunoadsorption in patients with Escherichia coli O104:H4-associated haemolytic uraemic syndrome: a prospective trial.免疫吸附法 IgG 清除治疗产志贺样毒素大肠埃希菌 O104:H4 相关性溶血尿毒综合征患者的严重神经功能缺损：一项前瞻性试验。

Lancet. 2011 Sep 24;378(9797):1166-73. doi: 10.1016/S0140-6736(11)61253-1. Epub 2011 Sep 2.

4

Prospective genomic characterization of the German enterohemorrhagic Escherichia coli O104:H4 outbreak by rapid next generation sequencing technology.采用快速下一代测序技术对德国肠出血性大肠杆菌 O104:H4 暴发进行前瞻性基因组特征分析。

PLoS One. 2011;6(7):e22751. doi: 10.1371/journal.pone.0022751. Epub 2011 Jul 20.

5

Early volume expansion during diarrhea and relative nephroprotection during subsequent hemolytic uremic syndrome.腹泻期间早期容量扩张及随后溶血尿毒综合征期间的相对肾保护作用。

Arch Pediatr Adolesc Med. 2011 Oct;165(10):884-9. doi: 10.1001/archpediatrics.2011.152. Epub 2011 Jul 22.

6

Identification of the Shiga toxin-producing Escherichia coli O104:H4 strain responsible for a food poisoning outbreak in Germany by PCR.通过聚合酶链式反应（PCR）鉴定引发德国食物中毒暴发的产志贺毒素大肠杆菌O104:H4菌株。

J Clin Microbiol. 2011 Sep;49(9):3439-40. doi: 10.1128/JCM.01312-11. Epub 2011 Jul 13.

7

Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study.2011 年德国一起溶血性尿毒综合征暴发相关大肠杆菌菌株的特征：一项微生物学研究。

Lancet Infect Dis. 2011 Sep;11(9):671-6. doi: 10.1016/S1473-3099(11)70165-7. Epub 2011 Jun 22.

8

Epidemic profile of Shiga-toxin-producing Escherichia coli O104:H4 outbreak in Germany.德国产志贺毒素大肠杆菌 O104:H4 疫情概况。

N Engl J Med. 2011 Nov 10;365(19):1771-80. doi: 10.1056/NEJMoa1106483. Epub 2011 Jun 22.

9

Shiga toxin, cytolethal distending toxin, and hemolysin repertoires in clinical Escherichia coli O91 isolates.临床分离的大肠杆菌O91菌株中的志贺毒素、细胞致死性膨胀毒素和溶血素组成情况

J Clin Microbiol. 2009 Jul;47(7):2061-6. doi: 10.1128/JCM.00201-09. Epub 2009 Apr 29.

10

Analysis of collection of hemolytic uremic syndrome-associated enterohemorrhagic Escherichia coli.溶血尿毒综合征相关肠出血性大肠杆菌的菌株分析

Emerg Infect Dis. 2008 Aug;14(8):1287-90. doi: 10.3201/eid1408.071082.

实时多重聚合酶链反应检测人粪便中产志贺毒素 2 的大肠杆菌 O104：H4。

Real-time multiplex PCR for detecting Shiga toxin 2-producing Escherichia coli O104:H4 in human stools.

机构信息

Institute for Hygiene and the National Consulting Laboratory for Hemolytic Uremic Syndrome, University of Münster, Münster, Germany.

出版信息

J Clin Microbiol. 2012 May;50(5):1752-4. doi: 10.1128/JCM.06817-11. Epub 2012 Feb 15.

DOI:10.1128/JCM.06817-11

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3347122/

Abstract

A real-time multiplex PCR targeting stx(2), wzy(O104), and fliC(H4) of enterohemorrhagic Escherichia coli (EHEC) O104:H4 correctly determined the presence or absence of these genes in 253 EHEC isolates and enrichment cultures of stool samples from 132 patients. It is a rapid, sensitive, and specific tool for detecting EHEC O104:H4 in human stools.

摘要

针对肠出血性大肠杆菌 (EHEC) O104:H4 的stx(2)、wzy(O104)和 fliC(H4)的实时多重 PCR 可准确确定 253 株 EHEC 分离株和 132 名患者粪便样本富集培养物中这些基因的存在或缺失情况。它是一种快速、敏感和特异的工具，可用于检测人粪便中的 EHEC O104:H4。