Department of Chemistry, Washington State University, Pullman, Washington 99164, USA.
Anal Chem. 2012 Apr 3;84(7):3231-9. doi: 10.1021/ac203116a. Epub 2012 Mar 13.
Monosaccharide structural isomers including sixteen methyl-D-glycopyranosides and four methyl-N-acetylhexosamines were subjected to ion mobility measurements by electrospray ion mobility mass spectrometry. Two ion mobility-MS systems were employed: atmospheric pressure drift tube ion mobility time-of-flight mass spectrometry and a Synapt G2 HDMS system which incorporates a low pressure traveling wave ion mobility separator. All the compounds were investigated as M + Na ions in the positive mode. A majority of the monosaccharide structural isomers exhibited different mobility drift times in either system, depending on differences in their anomeric and stereochemical configurations. In general, drift time patterns (relative drift times of isomers) matched between the two instruments. Higher resolving power was observed using the atmospheric pressure drift tube. Collision cross section values of monosaccharide structural isomers were directly calculated from the atmospheric pressure ion mobility experiments, and a collision cross section calibration curve was made for the traveling wave ion mobility instrument. Overall, it was demonstrated that ion mobility-mass spectrometry using either drift tube or traveling wave ion mobility is a valuable technique for resolving subtle variations in stereochemistry among the sodium adducts of monosaccharide methyl glycosides.
十六种甲基-D-吡喃糖苷和四种甲基-N-乙酰己糖胺的单糖结构异构体通过电喷雾离子淌度质谱进行了离子淌度测量。使用了两种离子淌度-MS 系统：大气压漂移管离子淌度飞行时间质谱和集成了低压行波离子淌度分离器的 Synapt G2 HDMS 系统。所有化合物均以正模式中的M+Na离子形式进行研究。大多数单糖结构异构体在两种系统中表现出不同的迁移率漂移时间，这取决于它们的端基和立体化学构型的差异。通常，两种仪器之间的漂移时间模式（异构体的相对漂移时间）相匹配。使用大气压漂移管观察到更高的分辨率。从大气压离子淌度实验中直接计算单糖结构异构体的碰撞截面值，并为行波离子淌度仪器制作了碰撞截面校准曲线。总的来说，结果表明，使用漂移管或行波离子淌度的离子淌度-质谱是一种有价值的技术，可以解析单糖甲基糖苷钠加合物中立体化学的细微变化。
