Chem Biol Drug Des. 2012 Jun;79(6):1049-55. doi: 10.1111/j.1747-0285.2012.01361.x. Epub 2012 Mar 19.
Plastrum Testudinis (PT) is often used as an important traditional Chinese medicine to treat bone diseases in China for centuries. To identify active components of PT involved in promoting proliferation of MSCs, PT was extracted with ethyl acetate and separated by silica gel column chromatography with gradient elution. Sixteen (Ts-1 ≈ 16) components were obtained, which were biologically evaluated by MTT assay and flow cytometry on the proliferation of rat marrow-derived mesenchymal stem cells (rMSCs). Results indicated that Ts-12 could induce the proliferation compared with control group (p < 0.05), while Ts-4 exhibited inhibitive effect. The chemical components of PT which regulated the proliferation of rMSCs were investigated by Gas Chromatography-Mass Spectrometry (GC-MS), HPLC, and nine standard compounds. The experimental results suggested that palmitic acid methyl ester and cholesterol myristate, which identified from Ts-12, possessed proliferative activity while stearic acid found in Ts-4 showed inhibition.
鳖甲（PT）在中国几个世纪以来一直被用作治疗骨病的重要中药。为了鉴定鳖甲中参与促进间充质干细胞增殖的活性成分，采用乙酸乙酯提取，硅胶柱层析梯度洗脱进行分离。得到 16 个（Ts-1≈16）成分，采用 MTT 法和流式细胞术检测它们对大鼠骨髓间充质干细胞（rMSCs）增殖的影响。结果表明 Ts-12 与对照组相比可诱导增殖（p<0.05），而 Ts-4 则表现出抑制作用。采用气相色谱-质谱联用（GC-MS）、高效液相色谱（HPLC）和 9 种标准化合物对调节 rMSCs 增殖的鳖甲化学成分进行了研究。实验结果表明，从 Ts-12 中鉴定出的棕榈酸甲酯和肉豆蔻酸胆固醇具有增殖活性，而在 Ts-4 中发现的硬脂酸则表现出抑制作用。
