Bours V, Villalobos J, Burd P R, Kelly K, Siebenlist U
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Nature. 1990 Nov 1;348(6296):76-80. doi: 10.1038/348076a0.
We have cloned and characterized a mitogen-inducible gene isolated from human T cells that predicts a protein of 968 amino acids. The amino-terminal domain has regions homologous to the oncogene rel and to the developmentally important gene dorsal of Drosophila. The carboxy-terminal domain contains repeat structures found in a variety of proteins that are involved in cell-cycle control of yeast and in tissue differentiation in Drosophila and Ceanorhabditis elegans, as well as in the putative human oncogene bcl-3 and in the ankyrin protein. A truncated form of the product of this gene translated in vitro is a DNA-binding protein which interacts specifically with the kappa B binding site found in many inducible genes, including the enhancer in human immunodeficiency virus. This gene is yet another in a growing list of important regulatory molecules whose expression is transcriptionally induced upon cellular activation.
我们已经克隆并鉴定了一个从人T细胞中分离出的促有丝分裂原诱导基因，该基因预测编码一种含968个氨基酸的蛋白质。氨基末端结构域有与癌基因rel以及果蝇发育中重要基因dorsal同源的区域。羧基末端结构域包含在多种蛋白质中发现的重复结构，这些蛋白质参与酵母的细胞周期调控、果蝇和秀丽隐杆线虫的组织分化，以及推定的人类癌基因bcl-3和锚蛋白。该基因产物的截短形式在体外翻译后是一种DNA结合蛋白，它能与许多诱导型基因（包括人类免疫缺陷病毒中的增强子）中发现的κB结合位点特异性相互作用。该基因是越来越多重要调控分子中的又一个，其表达在细胞活化时受到转录诱导。
