Product review. New mammalian expression vectors.

作者信息

Moss B, Elroy-Stein O, Mizukami T, Alexander W A, Fuerst T R

机构信息

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

Nature. 1990 Nov 1;348(6296):91-2. doi: 10.1038/348091a0.

DOI:10.1038/348091a0

PMID:2234068

Abstract

摘要

相似文献

Product review. New mammalian expression vectors.

Nature. 1990 Nov 1;348(6296):91-2. doi: 10.1038/348091a0.

Use of T7 RNA polymerase to direct expression of cloned genes.使用T7 RNA聚合酶指导克隆基因的表达。

Methods Enzymol. 1990;185:60-89. doi: 10.1016/0076-6879(90)85008-c.

[Vector system for gene expression based on the use of bacteriophage T7 RNA polymerase].

Dokl Akad Nauk SSSR. 1988;303(2):496-8.

[Cloning and study of the structural organization of regions of the inh(lip)-hoc genes of bacteriophage T4].[噬菌体T4 inh(lip)-hoc基因区域的结构组织克隆与研究]

Dokl Akad Nauk SSSR. 1988;303(6):1486-9.

[Synthetic promotor P25 of bacteriophage T5 and its functional hybrids with the model promotor of Escherichia coli].[噬菌体T5的合成启动子P25及其与大肠杆菌模型启动子的功能杂种]

Dokl Akad Nauk SSSR. 1984 Jan-Feb;274(1):213-6.

[Properties of cloned promoters].[克隆启动子的特性]

Mol Biol (Mosk). 1984 Jan-Feb;18(1):130-9.

Vectors for the synthesis of specific RNAs in vitro.用于体外合成特定RNA的载体。

Biotechnology. 1988;10:253-67. doi: 10.1016/b978-0-409-90042-2.50019-2.

A novel nonviral cytoplasmic gene expression system and its implications in cancer gene therapy.一种新型非病毒细胞质基因表达系统及其在癌症基因治疗中的意义。

Cancer Gene Ther. 1995 Dec;2(4):281-9.

[2 new promoters for RNA-polymerase of the phage SP6 are located beyond the gene for RNA-polymerase].

Dokl Akad Nauk SSSR. 1988;300(3):736-9.

[The structure of 2 promoters and a transcription terminator from the region of early genes D10-D15 of bacteriophage T5].[来自噬菌体T5早期基因D10 - D15区域的两个启动子和一个转录终止子的结构]

Mol Gen Mikrobiol Virusol. 1987 Oct(10):14-9.

引用本文的文献

Implementation of an Immunoassay Based on the MVA-T7pol-Expression System for Rapid Identification of Immunogenic SARS-CoV-2 Antigens: A Proof-of-Concept Study.基于 MVA-T7pol 表达系统的免疫测定法在快速鉴定 SARS-CoV-2 免疫原性抗原中的应用：概念验证研究。

Int J Mol Sci. 2024 Oct 10;25(20):10898. doi: 10.3390/ijms252010898.

Structural basis for dimerization of a paramyxovirus polymerase complex.副黏病毒聚合酶复合物二聚化的结构基础。

Nat Commun. 2024 Apr 11;15(1):3163. doi: 10.1038/s41467-024-47470-7.

Enhanced stability of the SARS CoV-2 spike glycoprotein following modification of an alanine cavity in the protein core.修饰蛋白核心中的丙氨酸空腔可增强 SARS-CoV-2 刺突糖蛋白的稳定性。

PLoS Pathog. 2023 May 18;19(5):e1010981. doi: 10.1371/journal.ppat.1010981. eCollection 2023 May.

Efficient production of protein complexes in mammalian cells using a poxvirus vector.利用痘病毒载体在哺乳动物细胞中高效生产蛋白复合物。

PLoS One. 2022 Dec 15;17(12):e0279038. doi: 10.1371/journal.pone.0279038. eCollection 2022.

Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene.从克隆 cDNA 中拯救一株肠致病性新城疫病毒 ZM10 株，并稳定表达插入的外源基因。

BMC Biotechnol. 2022 Dec 6;22(1):38. doi: 10.1186/s12896-022-00763-5.

Construction of Stable T7 Expression System in Saccharomyces cerevisiae by Improving Nuclear Membrane Permeability with Viroporin HIV-1 Vpu.利用 HIV-1 Vpu 提高核膜通透性构建酿酒酵母稳定的 T7 表达系统。

Appl Biochem Biotechnol. 2021 Dec;193(12):4214-4227. doi: 10.1007/s12010-021-03665-y. Epub 2021 Oct 11.

A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.登革病毒基因组 3' 末端序列在膜复制细胞器形成中的非复制作用。

Cell Rep. 2020 Jul 7;32(1):107859. doi: 10.1016/j.celrep.2020.107859.

A novel interaction between dengue virus nonstructural protein 1 and the NS4A-2K-4B precursor is required for viral RNA replication but not for formation of the membranous replication organelle.一种新发现的登革热病毒非结构蛋白 1 与 NS4A-2K-4B 前体之间的相互作用，对于病毒 RNA 复制是必需的，但对于膜复制细胞器的形成则不是必需的。

PLoS Pathog. 2019 May 9;15(5):e1007736. doi: 10.1371/journal.ppat.1007736. eCollection 2019 May.

M Segment-Based Minigenomes and Virus-Like Particle Assays as an Approach To Assess the Potential of Tick-Borne Genome Reassortment.基于 M 段的小基因组和病毒样颗粒测定作为评估蜱传基因组重配潜力的一种方法。

J Virol. 2019 Mar 5;93(6). doi: 10.1128/JVI.02068-18. Print 2019 Mar 15.

HCV NS5A dimer interface residues regulate HCV replication by controlling its self-interaction, hyperphosphorylation, subcellular localization and interaction with cyclophilin A.HCV NS5A 二聚体界面残基通过控制其自身相互作用、过度磷酸化、亚细胞定位以及与亲环素 A 的相互作用来调节 HCV 的复制。

PLoS Pathog. 2018 Jul 23;14(7):e1007177. doi: 10.1371/journal.ppat.1007177. eCollection 2018 Jul.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验