Department of Obstetrics and Gynecology, Charles University in Prague, Prague, Czech Republic.
Am J Obstet Gynecol. 2012 Apr;206(4):342.e1-8. doi: 10.1016/j.ajog.2012.01.004. Epub 2012 Jan 12.
The objective of the study was to determine whether the bacterial load of genital mycoplasmas and gestation age are related to intraamniotic inflammatory response using the amniotic fluid levels of 18 inflammatory mediators.
A prospective study of 145 women with singleton pregnancies complicated by preterm prelabor rupture of membranes between 24(0/7) and 36(6/7) weeks was conducted. Amniotic fluid was obtained from all women by transabdominal amniocentesis. The amounts of genital mycoplasma deoxyribonucleic acid were determined using the threshold cycle value and relative and absolute quantification techniques. A panel of multiple proteins was analyzed simultaneously using multiplex technology.
Twenty-four women with the presence of genital mycoplasmas in amniotic fluid were included in the final analyses. The concentrations of 9 of the 18 evaluated proteins in the amniotic fluid correlated with bacterial load of genital mycoplasmas independent of the quantification technique used.
The intensity of intraamniotic inflammatory response to genital mycoplasmas decreased with gestational age.
本研究旨在通过检测 18 种炎症介质在羊水中的水平，确定生殖支原体的细菌载量和妊娠年龄与羊膜内炎症反应之间的关系。
对 145 例单胎妊娠孕妇进行前瞻性研究，这些孕妇在 24（0/7）至 36（6/7）周时并发早产胎膜早破。所有孕妇均经腹羊膜穿刺术获取羊水。采用阈值循环值和相对及绝对定量技术检测生殖支原体脱氧核糖核酸含量。使用多重技术同时分析一组多种蛋白质。
最终分析纳入了 24 例羊水中存在生殖支原体的妇女。在使用不同定量技术的情况下，羊水 18 种评估蛋白中的 9 种蛋白的浓度与生殖支原体的细菌载量独立相关。
生殖支原体引起的羊膜内炎症反应的强度随妊娠年龄的增加而降低。
