Laboratório de Entomologia Forense, Departamento de Biologia Animal, Instituto de Biologia, UNICAMP, Cidade Universitária Zeferino Vaz s/n, CEP 13083-970, CP 6109 Campinas, São Paulo, Brazil.
Forensic Sci Int. 2012 Jul 10;220(1-3):27-32. doi: 10.1016/j.forsciint.2012.01.023. Epub 2012 Feb 15.
This study aimed to determine the effect of cocaine on the development and growth of immature and adult blowflies, in an attempt to better understand the impacts of such effects on postmortem interval (PMI) estimation. Twice the lethal dose of cocaine was injected into rabbits. The control animals were injected only with saline solution. Experimental and control rabbits were autopsied, and portions of their livers were exposed to newly eclosed larvae of Chrysomya putoria and Chrysomya albiceps. Larvae were weighed individually every 6 h, up 54 h of exposure. The larvae were then placed on an artificial diet to continue their development. Pupariation time, adult emergence and adult longevity were also analysed. The larvae of both species that fed on the cocaine-containing livers developed faster than those that fed on the livers of the control animals, leading to the conclusion that cocaine influences and stimulates larval growth. The difference in growth between the control and treated flies was best observed from 12 h of exposure onward. This finding has important implications for forensic investigations.
本研究旨在确定可卡因对未成年和成年蕈蚊发育和生长的影响，以期更好地了解这些影响对死后时间（PMI）推断的影响。向兔子注射两倍的致死剂量可卡因。对照动物仅注射生理盐水。对实验和对照兔子进行尸检，并将其部分肝脏暴露于新羽化的麻蝇和丝光绿蝇幼虫。幼虫在暴露的前 54 小时内，每 6 小时单独称重一次。然后将幼虫放在人工饲料上继续发育。蛹化时间、成虫出现和成虫寿命也进行了分析。两种蕈蚊的幼虫在含可卡因的肝脏上生长速度比在对照动物肝脏上生长的幼虫快，这表明可卡因会影响和刺激幼虫的生长。从暴露 12 小时开始，对照组和实验组苍蝇之间的生长差异最为明显。这一发现对法医学调查具有重要意义。
