Magni Paola A, Pacini Tommaso, Pazzi Marco, Vincenti Marco, Dadour Ian R
Centre for Forensic Science, University of Western Australia, Crawley 6009, Australia.
Department of Chemistry, University of Turin, Via Pietro Giuria 5, Torino 10125 Italy.
Forensic Sci Int. 2014 Aug;241:96-101. doi: 10.1016/j.forsciint.2014.05.004. Epub 2014 May 20.
Entomotoxicology is the study of using insects for the detection of drugs and other chemical substances in decomposing tissues. One research aspect in particular is the effects of these substances on arthropod development and morphology, and their consequences on the post mortem interval estimation. Since methamphetamine (MA) is becoming commonplace as an illegal recreational drug, a GC-MS method for the detection of MA in Calliphora vomitoria L. (Diptera: Calliphoridae) was developed and validated. Furthermore, the effect of MA on the development, growth rates and survival of the blowfly was investigated. Larvae were reared on liver substrates homogeneously spiked with measured amounts of MA (5 ng/g and 10 ng/g) based on typical concentrations found in human tissue in cases of death caused by MA overdose. The experimental results demonstrated that (i) MA produced a significant increase in the developmental time from egg to adult in C. vomitoria, (ii) approximately 60% of larvae exposed to either dose of MA died during the pupation period and (iii) the resultant lengths of larvae and pupae were on average significantly larger than the controls.
昆虫毒理学是研究利用昆虫检测腐败组织中的药物和其他化学物质。其中一个特别的研究方向是这些物质对节肢动物发育和形态的影响，以及它们对死后间隔时间估计的影响。由于甲基苯丙胺（MA）作为一种非法娱乐性药物变得越来越普遍，因此开发并验证了一种用于检测丽蝇（双翅目：丽蝇科）中MA的气相色谱 - 质谱联用（GC - MS）方法。此外，还研究了MA对丽蝇发育、生长速率和存活的影响。基于MA过量致死案例中人体组织中发现的典型浓度，在均匀添加了测量量MA（5 ng/g和10 ng/g）的肝脏底物上饲养幼虫。实验结果表明：（i）MA使丽蝇从卵到成虫的发育时间显著增加；（ii）暴露于任一剂量MA的幼虫中约60%在化蛹期死亡；（iii）幼虫和蛹的最终长度平均显著大于对照组。
