Department of Animal Biology, University of Campinas- UNICAMP, POB 6109, PC 13083-970, Campinas, São Paulo, Brazil,
Parasitol Res. 2014 May;113(5):1897-907. doi: 10.1007/s00436-014-3837-5. Epub 2014 Mar 15.
Entomotoxicology is focused on obtaining data on necrophagous entomofauna, for criminal investigations purposes. This study aimed to evaluate the effect of different concentrations of methylphenidate hydrochloride, phenobarbital, and their association on the developmental rate, larval and pupal survivorship, and the interval of emergence of adults of Chrysomya albiceps (Wiedemann), Chrysomya megacephala (Fabricius), and Chrysomya putoria (Wiedemann) (Diptera: Calliphoridae). Considering the therapeutic dose (TD) of methylphenidate hydrochloride (0.29 mg/Kg), the concentrations tested were 10× TD, 50× TD, and 100× TD. For phenobarbital, the concentrations used were 1× TD (=150 mg/Kg), 3.3× TD, and 6.7× TD. For the association of the drugs, the combinations used were 10× TD-methylphenidate hydrochloride plus 1× TD-phenobarbital, 50× TD-methylphenidate hydrochloride plus 3.3× TD-phenobarbital, and 100× TD-methylphenidate hydrochloride plus 6.7× TD-phenobarbital. The control group, without addition of drug, was maintained under the same conditions of temperature (25 ± 1 °C), humidity (70 ± 10%), and photoperiod (12 h). Specimens of each group were weighed every 12 h until pupariation. The developmental rate of the three Chrysomya species immatures was monitored. For C. albiceps the developmental time was delayed in 24 h for methylphenidate hydrochloride group and in 12 h for the phenobarbital and the drugs association groups. The effect was observed only at specific ages for C. megacephala, without altering the developmental time. For C. putoria, the developmental time was delayed in 12 h for methylphenidate hydrochloride group and in 24 h for the phenobarbital and the drugs association groups. The emergence interval was similar among all experimental groups, but larval and pupal viabilities were affected in different ways.
昆虫毒理学专注于获取有关腐生性昆虫区系的数据，以用于犯罪调查。本研究旨在评估不同浓度的盐酸哌甲酯、苯巴比妥及其联合使用对美洲大蠊（Wiedemann）、巨尾阿丽蝇（Fabricius）和黄胸丽蝇（Wiedemann）（双翅目：Calliphoridae）幼虫和蛹的存活率以及成虫出现间隔的影响。考虑到盐酸哌甲酯的治疗剂量（TD）（0.29mg/Kg），测试的浓度分别为 10×TD、50×TD 和 100×TD。对于苯巴比妥，使用的浓度为 1×TD（=150mg/Kg）、3.3×TD 和 6.7×TD。对于药物联合使用，使用的组合为 10×TD-盐酸哌甲酯加 1×TD-苯巴比妥、50×TD-盐酸哌甲酯加 3.3×TD-苯巴比妥和 100×TD-盐酸哌甲酯加 6.7×TD-苯巴比妥。未添加药物的对照组在相同的温度（25±1°C）、湿度（70±10%）和光照周期（12 h）条件下保持不变。每隔 12 h 对每组标本进行称重，直到蛹化。监测了三种丽蝇属昆虫幼虫的发育速度。对于 C. albiceps，盐酸哌甲酯组的发育时间延迟了 24 h，苯巴比妥组和药物联合组的发育时间延迟了 12 h。仅在特定年龄观察到对 C. megacephala 的影响，而不改变发育时间。对于 C. putoria，盐酸哌甲酯组的发育时间延迟了 12 h，苯巴比妥组和药物联合组的发育时间延迟了 24 h。各实验组的出现间隔相似，但幼虫和蛹的存活率受到不同方式的影响。
