College of Chemistry and Chemical Engineering, Graduate University of Chinese Academy of Sciences, Beijing, China.
Biosens Bioelectron. 2012 Apr 15;34(1):112-7. doi: 10.1016/j.bios.2012.01.026. Epub 2012 Jan 28.
A highly sensitive method for the detection of trace amount of clenbuterol based on gold nanoparticles (AuNPs) in the presence of melamine was described in this paper. Hydrogen-bonding interaction between clenbuterol and melamine resulted in the aggregation of AuNPs and a consequent color change of AuNPs from wine red to blue. The concentration of clenbuterol could be determined with naked eye or a UV-vis spectrometer. Results showed that the absorption ratio (A(670)/A(520)) was liner with the logarithm of clenbuterol concentration in the range of 2.8×10(-10) to 2.8×10(-7)M and 2.8×10(-7) to 1.4×10(-6)M with linear coefficients of 0.996 and 0.993, respectively. The detection limit was 2.8×10(-11)M (S/N=3), which was much lower than most existing methods. The coexisting substances including dl-epinephrine, phenylalamine, tryptohan, alamine, uric acid, glycine, glycerol, glucose, MgCl(2), CaCl(2) and NaCl did not affect the determination of clenbuterol. The proposed method could be successfully applied to the determination of clenbuterol in human urine.
本文描述了一种基于金纳米粒子（AuNPs）在三聚氰胺存在下检测痕量克伦特罗的高灵敏度方法。克伦特罗与三聚氰胺之间的氢键相互作用导致 AuNPs 的聚集，从而导致 AuNPs 的颜色从酒红色变为蓝色。克伦特罗的浓度可以用肉眼或紫外可见分光光度计来确定。结果表明，在 2.8×10(-10) 到 2.8×10(-7)M 和 2.8×10(-7) 到 1.4×10(-6)M 的范围内，吸光度比（A(670)/A(520)）与克伦特罗浓度的对数呈线性关系，线性系数分别为 0.996 和 0.993。检测限为 2.8×10(-11)M（S/N=3），远低于大多数现有方法。共存物质，包括 dl-肾上腺素、苯丙氨酸、色氨酸、丙氨酸、尿酸、甘氨酸、甘油、葡萄糖、MgCl(2)、CaCl(2) 和 NaCl，不影响克伦特罗的测定。该方法可成功应用于人尿中克伦特罗的测定。
