Department of Forensic Science, School of Sciences, Gujarat University, Ahmedabad, Gujarat-380009, India.
Analyst. 2013 Sep 21;138(18):5411-6. doi: 10.1039/c3an00184a. Epub 2013 Jun 27.
Inexpensive, rapid, and reliable methods of detection are crucial to the control of toxicological investigations. Here we report a highly sensitive, selective and cost effective method for the detection of trace amounts of clonazepam based on gold nanoparticles (AuNPs) in the presence of melamine. Hydrogen bonding interactions between clonazepam and melamine resulted in the aggregation of AuNPs and a consequent color change of AuNPs from wine red to blue. The results showed that the absorption ratio (A636/A552) was linear for clonazepam concentrations in the range of 10 × 10(-8) to 1.0 × 10(-9) M (R(2) = 0.999). The detection limit was 8.9 × 10(-10) M (S/N = 3), which was much lower than that of most existing methods. Coexisting substances including alprazolam, diazepam, nitrazepam and lorazepam did not affect the determination of clonazepam. The sensor developed by this new approach could be used as a spot test and a good alternative means for on-site and real time screening of clonazepam. This proposed scheme was also supported by the use of real samples such as skeletal remains and blood to illustrate the applicability of the developed nanosensor by a series of experiments.
廉价、快速、可靠的检测方法对于毒理学调查的控制至关重要。在这里，我们报告了一种基于金纳米粒子（AuNPs）在三聚氰胺存在下检测痕量氯硝西泮的高灵敏度、选择性和经济有效的方法。氯硝西泮与三聚氰胺之间的氢键相互作用导致 AuNPs 的聚集，从而导致 AuNPs 的颜色从酒红色变为蓝色。结果表明，在 10×10(-8)至 1.0×10(-9) M 的氯硝西泮浓度范围内，吸收比（A636/A552）呈线性（R(2) = 0.999）。检测限为 8.9×10(-10) M（S/N = 3），远低于大多数现有方法。包括阿普唑仑、地西泮、硝西泮和劳拉西泮在内的共存物质不会影响氯硝西泮的测定。通过这种新方法开发的传感器可作为现场即时筛查氯硝西泮的现场测试和良好替代手段。通过一系列实验，还使用骨骼遗骸和血液等实际样本来支持所提出的方案，说明了所开发的纳米传感器的适用性。
