Liu Xigui, Huang Danlian, Lai Cui, Wang Fei
Institute of Chemical Engineering, Guangdong Academy of Sciences, Guangzhou, 510665, People's Republic of China.
College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha, 410082, Hunan, People's Republic of China.
Mikrochim Acta. 2025 Jan 14;192(2):78. doi: 10.1007/s00604-025-06954-z.
A simple and rapid colorimetric detection strategy, based on hydrogen bond identification of 6-thioguanine (6-TG) functionalized Au nanoparticles (AuNPs), is proposed for highly selective and sensitive determination of kanamycin (KA). In this strategy, the hydrogen bond interaction between 6-TG and kanamycin induces AuNPs to agglomerate, with a consequent color change of AuNPs from wine red to purple or even blue. The kanamycin concentrations can be quantified by employing UV-vis spectrophotometer. The results display that kanamycin concentrations (0.005 to 18 µM) are linearly related to A/A (the absorbance ratio of 620 nm and 520 nm) with a LOD of 1.8 nM and a LOQ of 5.9 nM (S/N = 3). This strategy also reveals a high degree of selectivity among a series of common interfering species. Moreover, the strategy can be employed to detect trace amounts of kanamycin in real-life samples, and it shows satisfying results compared with high performance liquid chromatography. In general, this developed strategy is facile and inexpensive without the need for complex processing procedures and expensive instruments. In addition, this work may further exploit detection strategies for other organic contaminants, as well as make a strong contribution to the development of the colorimetric method.
提出了一种基于6-硫鸟嘌呤（6-TG）功能化金纳米颗粒（AuNPs）氢键识别的简单快速比色检测策略，用于高选择性和灵敏地测定卡那霉素（KA）。在该策略中，6-TG与卡那霉素之间的氢键相互作用诱导AuNPs团聚，导致AuNPs颜色从酒红色变为紫色甚至蓝色。卡那霉素浓度可通过紫外可见分光光度计进行定量。结果表明，卡那霉素浓度（0.005至18 μM）与A/A（620 nm和520 nm处的吸光度比值）呈线性关系，检测限为1.8 nM，定量限为5.9 nM（S/N = 3）。该策略在一系列常见干扰物质中也显示出高度的选择性。此外，该策略可用于检测实际样品中的痕量卡那霉素，与高效液相色谱法相比，结果令人满意。总体而言，这种开发的策略简便且成本低廉，无需复杂的处理程序和昂贵的仪器。此外，这项工作可能会进一步开拓其他有机污染物的检测策略，并为比色法的发展做出重要贡献。
