Department of Neurology, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006 Jiangsu, China.
J Clin Neurosci. 2012 May;19(5):750-2. doi: 10.1016/j.jocn.2011.08.033. Epub 2012 Feb 18.
Migraine is a debilitating condition characterized by a multi-factorial and polygenic mode of inheritance. Tumor necrosis factor alpha (TNF-α), a pro-inflammatory cytokine, has been implicated in the pathophysiological mechanisms of migraine. The aim of this study was to investigate whether TNF receptor superfamily member 1b gene (TNFRSF1B) polymorphism affects the occurrence of migraine in the Chinese Han population. We focused on a 15 base pair (bp) insertion/deletion polymorphism (rs5745946) in the 5' promoter region of TNFRSF1B, and investigated whether TNFRSF1B polymorphism is associated with susceptibility to migraine. We studied the genotypic and allelic frequencies of TNFRSF1B polymorphism in 416 patients with migraine and 415 control participants using polymerase chain reaction amplification and polyacrylamide gel electrophoresis analyses. The TNFRSF1B 15 bp insertion allele was associated with an increased risk for migraine (p=0.04, odds ratio=0.79, 95% confidence interval: 0.63-1.00). Our data suggested that individuals in the Chinese Han population with the TNFRSF1B 15 bp insertion allele may be at higher risk for migraine, which warrants further replication association studies and follow-up functional experiments.
偏头痛是一种使人虚弱的疾病，其遗传模式具有多因素和多基因的特点。肿瘤坏死因子-α（TNF-α）是一种促炎细胞因子，与偏头痛的病理生理机制有关。本研究旨在探讨 TNF 受体超家族成员 1b 基因（TNFRSF1B）多态性是否影响汉族人群偏头痛的发生。我们关注 TNFRSF1B 5'启动子区域的 15 个碱基对（bp）插入/缺失多态性（rs5745946），并研究 TNFRSF1B 多态性是否与偏头痛易感性相关。我们使用聚合酶链反应扩增和聚丙烯酰胺凝胶电泳分析，研究了 416 例偏头痛患者和 415 例对照参与者中 TNFRSF1B 多态性的基因型和等位基因频率。TNFRSF1B 15 bp 插入等位基因与偏头痛风险增加相关（p=0.04，比值比=0.79，95%置信区间：0.63-1.00）。我们的数据表明，汉族人群中携带 TNFRSF1B 15 bp 插入等位基因的个体可能患偏头痛的风险更高，这需要进一步进行复制关联研究和后续的功能实验。
