Mårtensson L, Blomqvist S, Jahr J, Thörne J
Department of Medical Research, University Hospital Lund, Sweden.
Nephron. 1990;56(1):86-91. doi: 10.1159/000186106.
Nine anesthetized pigs were subjected to short (90 min) sham dialysis with blood membrane contact with the aim to select effects of the artificial surface during dialysis. The importance of the neutrophil (PMN) was investigated by the selective isotope labelling, dynamically followed by gamma-camera imaging and biochemical assays specifically oriented for PMN function. These assays included cell count, PMN aggregation, PMN luminescence, fibronectin and catalase activity. Additionally, pulmonary and systemic hemodynamics and acid base balance were monitored. Sham dialysis induced an accumulation of labelled PMN attaining a maximum between 15 and 17 min. This was coupled with a time-related neutropenia, pulmonary vasoconstriction, increased in vitro PMN aggregation and luminescence response. The neutrophil response abated by the end of dialysis. Cardiac output and arterial blood pressure declined to a steady level after 30 min of sham dialysis. There was an insignificant decrease in catalase activity. All other parameters remained unaltered. The results indicate that PMN accumulates in the pulmonary vessels, in association with neutropenia and activation. The transience of the event points to a protective mechanisms of humoral and/or cellular character.
九只麻醉猪接受了为期90分钟的短期假透析，透析过程中血液与膜接触，目的是筛选透析过程中人工表面的影响。通过选择性同位素标记研究中性粒细胞（PMN）的重要性，随后通过γ相机成像和专门针对PMN功能的生化检测进行动态跟踪。这些检测包括细胞计数、PMN聚集、PMN发光、纤连蛋白和过氧化氢酶活性。此外，还监测了肺和全身血流动力学以及酸碱平衡。假透析导致标记的PMN积聚，在15至17分钟之间达到最大值。这与时间相关的中性粒细胞减少、肺血管收缩、体外PMN聚集增加和发光反应相关。透析结束时中性粒细胞反应减弱。假透析30分钟后，心输出量和动脉血压降至稳定水平。过氧化氢酶活性有轻微下降。所有其他参数均未改变。结果表明，PMN在肺血管中积聚，同时伴有中性粒细胞减少和激活。该事件的短暂性表明存在体液和/或细胞性质的保护机制。
