Allergy Research Foundation, Effenseweg 42, Breda, The Netherlands.
Respiration. 2012;84(5):385-95. doi: 10.1159/000335258. Epub 2012 Feb 14.
Patients with allergic bronchial asthma can develop various types of asthmatic response to bronchial challenge with allergens, such as immediate (IAR), late (LAR) or delayed (DYAR) response, due to different immunological mechanisms.
The DYAR, beginning within 26- 32 h after the challenge, reaching its maximum between 32 and 48 h and resolving within 56 h (p < 0.001), differs from IAR and LAR regarding the clinical features, diagnostic and immunological parameters.
The repeated DYAR (p < 0.001) in 28 patients was supplemented with recording of cytokine concentrations in the serum before and up to 72 h after the bronchial challenge by means of enzyme-linked immunoassay.
The DYAR was accompanied by a significant increase in the serum concentration (p < 0.05) of IL-2 at 24, 36 and 48 h; IL-10 at 12, 24, 36 and 48 h; IL-18 at 12 and 24 h; IFN-γ at 24, 36, 48 and 56 h; G-CSF at 1 and 12 h; TNF-α at 12, 36 and 48 h and TGF-β at 12 and 36 h, and a significant decrease in the concentration (p < 0.05) of IL-7 at 36 and 48 h and IL-12p70 at 12 h, as compared both with the prechallenge and with PBS control values. No significant changes in the serum cytokines were recorded during the PBS controls (p > 0.2).
These results support the evidence for an active involvement of the Th(1) cells, neutrophils, monocytes, macrophages, epithelial cells and endothelial cells, upon cooperation of other cell types, in the immunological mechanism(s) underlying the DYAR.
由于不同的免疫机制，患有过敏性支气管哮喘的患者在受到过敏原支气管激发后，可能会产生各种类型的哮喘反应，如即刻（IAR）、晚期（LAR）或延迟（DYAR）反应。
与 IAR 和 LAR 相比，于激发后 26-32 小时开始、32-48 小时达高峰、56 小时内缓解（p<0.001）的 DYAR 在临床特征、诊断和免疫参数方面存在差异。
通过酶联免疫吸附法，对 28 例患者的重复 DYAR（p<0.001）进行检测，并在支气管激发前及激发后 72 小时内记录血清细胞因子浓度。
DYAR 时，血清中 IL-2 在 24、36 和 48 小时时浓度显著增加（p<0.05）；IL-10 在 12、24、36 和 48 小时时浓度显著增加；IL-18 在 12 和 24 小时时浓度显著增加；IFN-γ 在 24、36、48 和 56 小时时浓度显著增加；G-CSF 在 1 和 12 小时时浓度显著增加；TNF-α 在 12、36 和 48 小时时浓度显著增加；TGF-β 在 12 和 36 小时时浓度显著增加，而 IL-7 在 36 和 48 小时时浓度和 IL-12p70 在 12 小时时浓度显著降低（p<0.05），与激发前和 PBS 对照值相比。在 PBS 对照中，血清细胞因子没有显著变化（p>0.2）。
这些结果支持了 Th(1)细胞、中性粒细胞、单核细胞、巨噬细胞、上皮细胞和内皮细胞通过与其他细胞类型的合作，积极参与 DYAR 背后的免疫机制的证据。
