用于定量分析新型抗精神病药物阿立哌唑及其两种主要人体尿液代谢物的自动固相萃取-高效液相色谱-串联质谱法的开发与验证
Development and validation of automated SPE-HPLC-MS/MS methods for the quantification of asenapine, a new antipsychotic agent, and its two major metabolites in human urine.
作者信息
de Boer Theo, Meulman Erik, Meijering Henri, Wieling Jaap, Dogterom Peter, Lass Holger
机构信息
QPS Netherlands, Petrus Campersingel 123, 9713, AG, Groningen, The Netherlands.
出版信息
Biomed Chromatogr. 2012 Dec;26(12):1461-3. doi: 10.1002/bmc.2722. Epub 2012 Feb 16.
To support the evaluation of the pharmacokinetic parameters of asenapine (ASE) in urine, we developed and validated online solid-phase extraction high-performance liquid chromatography methods with tandem mass spectrometry detection (SPE-LC-MS/MS) for the quantification of ASE and two of its major metabolites, N-desmethylasenapine (DMA) and asenapine-N⁺-glucuronide (ASG). The linearity in human urine was found acceptable for quantification in a concentration range of 0.500-100 ng/mL for ASE and DMA and 10.0-3000 ng/mL for ASG, respectively.
为支持对阿塞那平（ASE）在尿液中的药代动力学参数进行评估，我们开发并验证了在线固相萃取-高效液相色谱-串联质谱检测法（SPE-LC-MS/MS），用于定量分析ASE及其两种主要代谢产物N-去甲基阿塞那平（DMA）和阿塞那平-N⁺-葡萄糖醛酸苷（ASG）。结果发现，在人尿液中，ASE和DMA浓度范围为0.500 - 100 ng/mL、ASG浓度范围为10.0 - 3000 ng/mL时，定量分析的线性度可接受。
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