de Boer Theo, Meulman Erik, Meijering Henri, Wieling Jaap, Dogterom Peter, Lass Holger
QPS Netherlands, Petrus Campersingel 123, 9713, AG, Groningen, The Netherlands.
Biomed Chromatogr. 2012 Dec;26(12):1461-3. doi: 10.1002/bmc.2722. Epub 2012 Feb 16.
To support the evaluation of the pharmacokinetic parameters of asenapine (ASE) in urine, we developed and validated online solid-phase extraction high-performance liquid chromatography methods with tandem mass spectrometry detection (SPE-LC-MS/MS) for the quantification of ASE and two of its major metabolites, N-desmethylasenapine (DMA) and asenapine-N⁺-glucuronide (ASG). The linearity in human urine was found acceptable for quantification in a concentration range of 0.500-100 ng/mL for ASE and DMA and 10.0-3000 ng/mL for ASG, respectively.
为支持对阿塞那平（ASE）在尿液中的药代动力学参数进行评估，我们开发并验证了在线固相萃取-高效液相色谱-串联质谱检测法（SPE-LC-MS/MS），用于定量分析ASE及其两种主要代谢产物N-去甲基阿塞那平（DMA）和阿塞那平-N⁺-葡萄糖醛酸苷（ASG）。结果发现，在人尿液中，ASE和DMA浓度范围为0.500 - 100 ng/mL、ASG浓度范围为10.0 - 3000 ng/mL时，定量分析的线性度可接受。
