鉴定新型解淀粉芽孢杆菌 E33L 基因 ampD 编码的溶菌蛋白为炭疽杆菌抗菌蛋白。
Characterization of a novel lytic protein encoded by the Bacillus cereus E33L gene ampD as a Bacillus anthracis antimicrobial protein.
机构信息
Biosciences and Biotechnology Division, Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, California, USA.
出版信息
Appl Environ Microbiol. 2012 Apr;78(8):3025-7. doi: 10.1128/AEM.06906-11. Epub 2012 Feb 17.
Abstract
Lytic proteins encoded by bacterial genomes have been implicated in cell wall biosynthesis and recycling. The Bacillus cereus E33L ampD gene encodes a putative N-acetylmuramoyl-l-alanine amidase. This gene, expressed in vitro, produced a very stable, highly active lytic protein. Very low concentrations rapidly and efficiently lyse vegetative Bacillus anthracis cells.
摘要
细菌基因组编码的溶菌蛋白与细胞壁的生物合成和循环有关。蜡样芽胞杆菌 E33L ampD 基因编码一种假定的 N-乙酰胞壁酰-L-丙氨酸酰胺酶。该基因在体外表达产生一种非常稳定、高活性的溶菌蛋白。极低浓度即可快速有效地裂解有活力的炭疽杆菌细胞。
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