Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University, Shenyang, PR China.
Mol Med Rep. 2012 May;5(5):1277-80. doi: 10.3892/mmr.2012.794. Epub 2012 Feb 16.
Ovarian cancer affects females worldwide and is associated with poor patient prognosis. Identification of diagnostic biomarkers and effective therapeutic targets is vital for the diagnosis and treatment of ovarian cancer. Recently, researchers have found that microRNAs play several important roles in carcinogenesis. The purpose of this study was to investigate the relationship between miR-148b expression in human ovarian cancer tissues and clinicopathological features. Seventy-seven ovarian carcinoma tissues and 17 normal ovary tissues were collected from the First Hospital of China Medical University. For quantitative detection of the expression levels of miR-148b, total RNA was extracted and then reverse transcription-polymerase chain reaction was performed. The relationship between miR-148b expression in ovarian cancer and clinicopathological features was analyzed. We found that miR-148b was overexpressed in 92.21% (71/77) of the ovarian cancer samples examined, and overexpression of miR-148b in ovarian cancer tissues was not associated with any of the clinicopathological features of patients with ovarian cancer. Taken together, miR-148b may be involved in the early stage of ovarian carcinogenesis and could be used as an efficient diagnostic biomarker.
卵巢癌影响全球女性，与患者预后不良有关。鉴定诊断生物标志物和有效的治疗靶点对于卵巢癌的诊断和治疗至关重要。最近，研究人员发现 microRNAs 在致癌作用中发挥着多种重要作用。本研究旨在探讨人卵巢癌组织中 miR-148b 表达与临床病理特征之间的关系。本研究从中国医科大学第一医院收集了 77 例卵巢癌组织和 17 例正常卵巢组织。为了定量检测 miR-148b 的表达水平，提取了总 RNA，然后进行了逆转录-聚合酶链反应。分析了 miR-148b 在卵巢癌中的表达与临床病理特征之间的关系。我们发现，在所检查的 77 例卵巢癌样本中，miR-148b 表达过度，占 92.21%（71/77），miR-148b 在卵巢癌组织中的过度表达与卵巢癌患者的任何临床病理特征均无关。总之，miR-148b 可能参与了卵巢癌的早期发生过程，可作为一种有效的诊断生物标志物。
