Tian Songyu, Zhang Mingyue, Chen Xiuwei, Liu Yunduo, Lou Ge
Department of Gynecology Oncology, Cancer Hospital of Harbin Medical University, Harin, 150081, Heilongjiang, China.
Department of Anaesthesiology, Cancer Hospital of Harbin Medical University, Harin, 150081, Heilongjiang, China.
Oncotarget. 2016 Dec 27;7(52):87091-87099. doi: 10.18632/oncotarget.13526.
Ovarian cancer is among the leading cause of cancer-related deaths in females. In this study, we demonstrated that miR-595 expression was downregulated in the ovarian cancer tissues and cell lines. miR-595 expression was lower in the lymph node metastases tissues than in the primary ovarian cancer tissues and normal tissues. Furthermore, miR-595 overexpression suppressed the ovarian cancer cell proliferation, colony formation and invasion and promoted the sensitivity of ovarian cancer cell to cisplatin. We identified ABCB1 as a direct target gene of miR-595 in the ovarian cancer cell. ABCB1 expression was upregulated in the ovarian cancer tissues and cell lines. Morevoer, the expression level of ABCB1 was inversely correlated with miR-595 in the ovarian cancer tissues. In addition, overexpression of ABCB1 decreased the miR-595-overexpressing HO8910PM and SKOV-3 cell sensitivity to cisplatin. Ectopic expression of ABCB1 promoted the miR-595-overexpressing HO8910PM and SKOV-3 cell proliferation, colony formation and invasion. These data suggested that miR-595 acted a tumor suppressor role in ovarian cancer development and increased the sensitivity of ovarian cancer to cisplatin.
卵巢癌是女性癌症相关死亡的主要原因之一。在本研究中，我们证明miR-595在卵巢癌组织和细胞系中的表达下调。miR-595在淋巴结转移组织中的表达低于原发性卵巢癌组织和正常组织。此外，miR-595过表达抑制卵巢癌细胞增殖、集落形成和侵袭，并提高卵巢癌细胞对顺铂的敏感性。我们确定ABCB1是卵巢癌细胞中miR-595的直接靶基因。ABCB1在卵巢癌组织和细胞系中的表达上调。此外，在卵巢癌组织中ABCB1的表达水平与miR-595呈负相关。另外，ABCB1过表达降低了miR-595过表达的HO8910PM和SKOV-3细胞对顺铂的敏感性。ABCB1的异位表达促进了miR-595过表达的HO8910PM和SKOV-3细胞的增殖、集落形成和侵袭。这些数据表明miR-595在卵巢癌发展中起肿瘤抑制作用，并增加了卵巢癌对顺铂的敏感性。
