Zhu Lin, Han Jian-Bao, Zhang Xi-He, Ma Jian-Ping, Lv Long-Bao, Zhang Gao-Hong, Zheng Yong-Tang
Chinese Academy of Sciences, Kunming, China.
Dongwuxue Yanjiu. 2012 Feb;33(1):49-54. doi: 10.3724/SP.J.1141.2012.01049.
Nonhuman primates are critical resources for biomedical research. Rhesus macaque is a popularly used laboratory nonhuman primate that share many characteristics with humans. However, rhesus macaques are the natural host of two exogenous retroviruses, SRV (simian type D retrovirus) and STLV (simian T lymphotropic virus). SRV and STLV may introduce potentially significant confounding factors into the study of AIDS model. Moreover, B virus (ceropithecine herpesvirus 1) is likely to harm not only rhesus macaque but also humans in experiments involving rhesus macaque. Yunnan province has large-scale breeding colonies of Chinese rhesus macaque. Therefore there is an urgent need for SPF Chinese rhesus macaque colonies. Here we investigated SRV, STLV and BV infections in 411 Chinese rhesus macaque by PCR technique. The results showed that the prevalence of SRV, STLV and BV among Chinese rhesus macaque breeding colony was 19.71% (81/411), 13.38% (55/411) and 23.11% (95/411), respectively. Comparison of viruses infection in different age-groups and male/female of Chinese rhesus macaque was also analyzed. This study will contribute to establishment of SPF Chinese rhesus macaque breeding colony.
非人灵长类动物是生物医学研究的关键资源。恒河猴是一种广泛使用的实验用非人灵长类动物，与人类有许多共同特征。然而，恒河猴是两种外源性逆转录病毒SRV（猴D型逆转录病毒）和STLV（猴嗜T淋巴细胞病毒）的天然宿主。SRV和STLV可能会给艾滋病模型研究引入潜在的重大混杂因素。此外，在涉及恒河猴的实验中，B病毒（猕猴疱疹病毒1型）不仅可能危害恒河猴，还可能危害人类。云南省有大规模的中国恒河猴繁殖群体。因此，迫切需要建立无特定病原体（SPF）中国恒河猴群体。在此，我们采用PCR技术对411只中国恒河猴的SRV、STLV和BV感染情况进行了调查。结果显示，中国恒河猴繁殖群体中SRV、STLV和BV的感染率分别为19.71%（81/411）、13.38%（55/411）和23.11%（95/411）。我们还分析了中国恒河猴不同年龄组和不同性别之间病毒感染情况的比较。本研究将有助于建立无特定病原体中国恒河猴繁殖群体。
