Microbial Biotechnology Research Laboratory, Department of Biochemistry, Manipur University, Canchipur, Imphal, 795003 Manipur, India.
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):114-118. doi: 10.1099/ijs.0.036087-0. Epub 2012 Feb 17.
A novel actinobacterial strain, MBRL 353(T), was isolated from a sample collected from a limestone quarry at Hundung, Manipur, India. Comparison of 16S rRNA gene sequences of strain MBRL 353(T) and other members of the genus Rhodococcus showed sequence similarities ranging from 95.5 to 98.2 %, with strain MBRL 353(T) showing closest sequence similarity to Rhodococcus triatomae IMMIB RIV-085(T) (98.2 %) and Rhodococcus equi DSM 20307(T) (97.2 %). DNA-DNA hybridization results, however, revealed that DNA-DNA relatedness values between strain MBRL 353(T) and R. triatomae DSM 44892(T) (43.4 %) and R. equi DSM 20307(T) (33.4 %) were well below the 70 % limit for species identification. Strain MBRL 353(T) contained meso-diaminopimelic acid as the diagnostic diamino acid and galactose and arabinose in the cell wall. Mycolic acids were present. The major fatty acids were C(16 : 0) (45.7 %), C(18 : 1)ω9c (18.2 %) and 10-methyl C(18 : 0) (11.3 %). The only menaquinone detected was MK-8(H(2)), while the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unknown phospholipid. The G+C content of the genomic DNA was 69.2 mol%. The phenotypic and genotypic data showed that strain MBRL 353(T) merits recognition as a representative of a novel species of the genus Rhodococcus for which the name Rhodococcus canchipurensis sp. nov. is proposed; the type strain is MBRL 353(T) (= KCTC 19851(T) = JCM 17578(T)).
一株新的放线菌菌株，MBRL 353(T)，从印度曼尼普尔邦 Hundung 的一个石灰岩采石场采集的样本中分离得到。MBRL 353(T)菌株与棒状杆菌属的其他成员的 16S rRNA 基因序列比较显示，序列相似性范围为 95.5%至 98.2%，与 MBRL 353(T)菌株最相似的是三锥虫 Rhodococcus triatomae IMMIB RIV-085(T)（98.2%）和马红球菌 Rhodococcus equi DSM 20307(T)（97.2%）。然而，DNA-DNA 杂交结果表明，MBRL 353(T)菌株与三锥虫 Rhodococcus triatomae DSM 44892(T)（43.4%）和马红球菌 Rhodococcus equi DSM 20307(T)（33.4%）之间的 DNA-DNA 相关值远低于种间识别的 70%界限。MBRL 353(T)菌株含有 meso-二氨基庚二酸作为诊断性二氨基庚二酸，细胞壁中含有半乳糖和阿拉伯糖。存在类脂酸。主要脂肪酸为 C(16:0)（45.7%）、C(18:1)ω9c（18.2%）和 10-甲基 C(18:0)（11.3%）。检测到的唯一menaquinone 是 MK-8(H(2))，而主要的极性脂质是双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和一种未知的磷脂。基因组 DNA 的 G+C 含量为 69.2mol%。表型和基因型数据表明，MBRL 353(T)菌株是棒状杆菌属的一个新种的代表，因此建议将其命名为 canchipurensis 新种 Rhodococcus canchipurensis sp. nov.；模式菌株是 MBRL 353(T)=KCTC 19851(T)=JCM 17578(T)。
