Centre for Drug Discovery and Development, Sathyabama University, Jeppiar Nagar, Chennai, 600 119, Tamil Nadu, India.
Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem, 636 011, Tamil Nadu, India.
Environ Sci Pollut Res Int. 2016 Jul;23(14):13832-42. doi: 10.1007/s11356-016-6532-5. Epub 2016 Mar 31.
An attempt has been made to isolate, purify and characterize antifouling compound from Streptomyces fradiae PE7 isolated from Vellar estuarine sediment, Parangipettai, South India. The microbial identification was done at species level based on its phenotypic, cell wall and molecular characteristics. Strain PE7 produced high quantity of antifouling compounds in agar surface fermentation when compared to submerged fermentation. In fermentation optimization, wide range of sugars, amino acids, minerals, pH, temperature and NaCl concentration was found to influence the antifouling compound production from the strain PE7. Antifouling compound PE7-C was purified from the crude extract by preparative TLC, and its activity against biofouling bacteria was confirmed by bioautography. Based on the physico-chemical characteristics, the chemical structure of the antifouling compound PE7-C was identified as quercetin (C15H10O7), a flavonoid class of compound with the molecular weight 302.23 g/mol. The purified quercetin was active against 18 biofouling bacteria with MIC range between 1.6 and 25 μg/ml, algal spore germination and mollusc foot adherence found at 100 μg/ml and 306 ± 19.6 μg ml(-1) respectively. The present study, for the first time, reported quercetin from marine-derived Streptomyces sp. PE7 with antifouling activity. This also leads to the repurposing of quercetin for the development of antifouling agent.
已经尝试从印度南部帕兰吉佩塔伊的 Vellar 河口沉积物中分离、纯化和表征来自链霉菌 fradiae PE7 的抗污化合物。根据其表型、细胞壁和分子特征，在种水平上进行了微生物鉴定。与深层发酵相比，PE7 菌株在琼脂表面发酵时产生了大量的抗污化合物。在发酵优化中，发现广泛的糖、氨基酸、矿物质、pH 值、温度和 NaCl 浓度都会影响 PE7 菌株的抗污化合物的产生。通过制备性 TLC 从粗提取物中纯化抗污化合物 PE7-C，并通过生物自显影法证实其对生物污损细菌的活性。根据理化特性，确定抗污化合物 PE7-C 的化学结构为槲皮素（C15H10O7），这是一种具有 302.23 g/mol 分子量的类黄酮化合物。纯化的槲皮素对 18 种生物污损细菌具有活性，MIC 范围在 1.6 和 25 μg/ml 之间，对藻类孢子萌发和贝类足部附着的活性分别在 100 μg/ml 和 306±19.6 μg/ml 左右。本研究首次从海洋来源的链霉菌 sp. PE7 中报道了具有抗污活性的槲皮素。这也导致了槲皮素在抗污剂开发中的重新应用。
