Microbial Biotechnology Research Laboratory, Department of Biochemistry, Manipur University, Canchipur, Imphal, 795003, Manipur, India.
Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4546-4551. doi: 10.1099/ijs.0.052746-0. Epub 2013 Aug 1.
Strain MBRL 34(T), isolated from a sample of limestone quarry located at Hundung, Manipur, India, was characterized by polyphasic taxonomy. The strain showed the highest 16S rRNA gene sequence similarity with Micromonospora echinaurantica DSM 43904(T) (98.4 %), but formed a monophyletic clade with Micromonospora coerulea DSM 43143(T) (98.3 %) in the neighbour-joining tree. DNA-DNA hybridization experiments gave a DNA-DNA relatedness value of 53.1 % between MBRL 34(T) and M. coerulea DSM 43143(T). Strain MBRL 34(T) contained meso-diaminopimelic acid, galactose and glucose in the whole-cell hydrolysates along with small amounts of mannose, xylose, rhamnose and ribose. The major polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside, along with an unknown lipid. MK-10(H6), MK-10(H2) MK-11(H4) and MK-10(H4) were the predominant menaquinones detected. The major fatty acids were iso-C16 : 0 and iso-C15 : 0. The G+C content of the genomic DNA was 73.5 %. Based on the taxonomic characteristics from a polyphasic study, strain MBRL 34(T) merits recognition as a representative of a novel species of the genus Micromonospora for which the name Micromonospora kangleipakensis sp. nov. is proposed; the type strain is MBRL 34(T) ( = DSM 45612(T) = JCM 17696(T)).
从印度曼尼普尔邦 Hundung 的石灰岩采石场样本中分离到的菌株 MBRL 34(T)，通过多相分类学进行了特征描述。该菌株与 Micromonospora echinaurantica DSM 43904(T) 的 16S rRNA 基因序列相似度最高（98.4%），但在系统发育树中与 Micromonospora coerulea DSM 43143(T) 形成一个单系分支（98.3%）。DNA-DNA 杂交实验得出 MBRL 34(T)与 M. coerulea DSM 43143(T)之间的 DNA-DNA 相关值为 53.1%。菌株 MBRL 34(T)的全细胞水解物中含有 meso-二氨基庚二酸、半乳糖和葡萄糖，以及少量的甘露糖、木糖、鼠李糖和核糖。检测到的主要极性脂类有二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷，以及一种未知脂质。检测到的主要醌类为 MK-10(H6)、MK-10(H2)、MK-11(H4)和 MK-10(H4)。主要脂肪酸为 iso-C16:0 和 iso-C15:0。基因组 DNA 的 G+C 含量为 73.5%。基于多相研究的分类特征，菌株 MBRL 34(T)值得作为 Micromonospora 属的一个新种的代表菌株加以确认，现提出 Micromonospora kangleipakensis sp. nov.的新种名；其模式菌株为 MBRL 34(T)（=DSM 45612(T)=JCM 17696(T)）。
