Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):109-113. doi: 10.1099/ijs.0.038547-0. Epub 2012 Feb 17.
A moderately thermophilic and strictly anaerobic bacterium, designated HS1(T), was isolated from offshore hot spring sediment in Xiamen, China. Cells were Gram-negative, catalase-positive, oxidase-negative, slender and flexible rods without flagella. The strain could grow at 35-55 °C (optimum at 50 °C) and in 1-8 % NaCl (w/v; optimum 2-4 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HS1(T) was affiliated with the family Marinilabiliaceae and shared a distant relationship with the previously described genera. The isolate was most closely related to Anaerophaga thermohalophila Fru22(T) with 16S rRNA gene sequence similarity of 92.4 %, followed by the other members of the family Marinilabiliaceae with 88.7-91.1 % similarity. The dominant cellular fatty acids were iso-C(15 : 0) and anteiso-C(15 : 0). The predominant quinone was MK-7. The major polar lipids were phosphatidylethanolamine (PE) and an unknown polar lipid. The genomic DNA G+C content was 38.7 mol%. Besides the phylogenetically distant relationship, strain HS1(T) was obviously distinguished from the most closely related genera in several phenotypic properties including colony colour and pigment production, optimal temperature, optimal NaCl, relation to O(2), bicarbonate/carbonate requirement, catalase activity, nitrate reduction, fermentation products and cellular fatty acid profile. Based on the phenotypic and phylogenetic data, strain HS1(T) represents a novel species of a new genus, for which the name Thermophagus xiamenensis gen. nov., sp. nov. is proposed. The type strain of the type species is HS1(T) (= DSM 19012(T) = CGMCCC 1.5071(T)).
一株中度嗜热、严格厌氧菌，命名为 HS1(T)，从中国厦门近海温泉沉积物中分离得到。细胞革兰氏阴性，过氧化氢酶阳性，氧化酶阴性，为细长灵活的无鞭毛杆菌。该菌株可在 35-55°C（最佳温度为 50°C）和 1-8%NaCl（w/v；最佳 2-4%）中生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 HS1(T)属于 Marinilabiliaceae 科，与先前描述的属关系较远。该分离株与 Anaerophaga thermohalophila Fru22(T)的 16S rRNA 基因序列相似性最高，为 92.4%，其次是 Marinilabiliaceae 科的其他成员，相似性为 88.7-91.1%。主要细胞脂肪酸为 iso-C(15:0)和 anteiso-C(15:0)。主要醌为 MK-7。主要极性脂为磷脂酰乙醇胺 (PE)和一种未知极性脂。基因组 DNA G+C 含量为 38.7mol%。除了系统发育上的亲缘关系外，菌株 HS1(T)在几个表型特性上与最密切相关的属明显不同，包括菌落颜色和色素产生、最适温度、最适 NaCl、与 O(2)的关系、碳酸氢盐/碳酸盐需求、过氧化氢酶活性、硝酸盐还原、发酵产物和细胞脂肪酸图谱。基于表型和系统发育数据，菌株 HS1(T)代表了一个新属的新种，建议将其命名为 Thermophagus xiamenensis gen. nov.，sp. nov.。模式种为 HS1(T)=DSM 19012(T)=CGMCCC 1.5071(T)。
