Nillawar Anup N, Bardapurkar J S, Bardapurkar S J
Department of Biochemistry, Government Medical College, Aurangabad, India.
Lung India. 2012 Jan;29(1):24-9. doi: 10.4103/0970-2113.92358.
Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease, mainly due to tobacco smoke. Pulmonary function tests (PFTs) are mandatory to diagnose COPD which shows irreversible airway obstruction. This study was aimed at understanding the behavior of biochemical parameters such as high sensitive C-reactive protein (hs-CRP) and lactate dehydrogenase (LDH) isoenzymes in COPD. Cytoplasmic cellular enzymes, such as LDH in the extracellular space, although of no further metabolic function in this space, are of benefit because they serve as indicators suggestive of disturbances of the cellular integrity induced by pathological conditions. The lung pattern is characterized by proportional increases in isoenzymes 3, 4, and 5. Hs-CRP indicates low grade of systemic inflammation.
Total (n = 45) patients of COPD (diagnosed on PFTs) were included. We followed the guidelines laid by the institute ethical committee. Investigations performed on the serum were the serum for hs-CRP, LDH isoenzymes on agarose gel electrophoresis.
The results obtained showed that the value of hs-CRP was 4.6 ± 0.42 mg/L. The isoenzymes pattern was characterized by an increase in LDH-3 and LDH-4 fractions. This is evident even in those patients with normal LDH (n = 13) levels.
This study states that there is a moderate positive correlation in between CRP and LDH-3 (r = 0.33; P = 0.01). Raised LDH-3 levels do not correlate with FEV(1) % (forced expiratory volume in first second) predicted. Moreover, it associates positively with hs-CRP and smoking status and negatively with body mass index. This underlines the potential of these parameters to complement the present system of staging which is solely based upon FEV(1) % predicted.
慢性阻塞性肺疾病（COPD）是一种慢性炎症性疾病，主要由烟草烟雾引起。肺功能测试（PFTs）是诊断显示不可逆气道阻塞的COPD所必需的。本研究旨在了解慢性阻塞性肺疾病中高敏C反应蛋白（hs-CRP）和乳酸脱氢酶（LDH）同工酶等生化参数的表现。细胞质细胞酶，如细胞外空间中的LDH，尽管在该空间中没有进一步的代谢功能，但因其可作为提示病理状况引起的细胞完整性紊乱的指标而具有重要意义。肺部模式的特征是同工酶3、4和5成比例增加。Hs-CRP表明存在低度全身炎症。
纳入了总共45例经肺功能测试确诊的COPD患者。我们遵循了机构伦理委员会制定的指导方针。对血清进行的检测包括用于检测hs-CRP的血清、琼脂糖凝胶电泳检测LDH同工酶。
获得的结果显示，hs-CRP的值为4.6±0.42mg/L。同工酶模式的特征是LDH-3和LDH-4组分增加。即使在那些LDH水平正常（n = 13）的患者中也是如此。
本研究表明，CRP与LDH-3之间存在中度正相关（r = 0.33；P = 0.01）。升高的LDH-3水平与预测的第一秒用力呼气量（FEV(1) %）无关。此外，它与hs-CRP和吸烟状况呈正相关，与体重指数呈负相关。这突出了这些参数补充目前仅基于预测的FEV(1) %的分期系统的潜力。
