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A procedure for the quantitation of relaxed closed circular DNA in the presence of superhelical DNA: an improved fluorometric assay for nicking-closing enzyme.

作者信息

Kowalski D

出版信息

Anal Biochem. 1979 Mar;93(2):346-54. doi: 10.1016/s0003-2697(79)80161-x.

DOI:10.1016/s0003-2697(79)80161-x
PMID:223466
Abstract
摘要

相似文献

1
A procedure for the quantitation of relaxed closed circular DNA in the presence of superhelical DNA: an improved fluorometric assay for nicking-closing enzyme.一种在超螺旋DNA存在下定量松弛闭环DNA的方法:一种改进的切口封闭酶荧光测定法。
Anal Biochem. 1979 Mar;93(2):346-54. doi: 10.1016/s0003-2697(79)80161-x.
2
DNA topoisomerase I-mediated nicking of circular duplex DNA.DNA拓扑异构酶I介导的环状双链DNA切口形成
Methods Mol Biol. 2001;95:81-7. doi: 10.1385/1-59259-057-8:81.
3
Fluorometric methods employing low concentrations of ethidium bromide for DNA topoisomerase and endonuclease assays.采用低浓度溴化乙锭进行DNA拓扑异构酶和核酸内切酶检测的荧光测定方法。
Anal Biochem. 1989 Nov 1;182(2):284-8. doi: 10.1016/0003-2697(89)90595-2.
4
Quantitation of ethidium-stained closed circular DNA in agarose gels.琼脂糖凝胶中溴化乙锭染色的闭环DNA定量分析。
J Biochem Biophys Methods. 1997 Feb 1;34(1):73-9. doi: 10.1016/s0165-022x(96)01204-3.
5
The effect of salt on the binding of the eucaryotic DNA nicking-closing enzyme to DNA and chromatin.盐对真核生物DNA切口封闭酶与DNA及染色质结合的影响。
Biochim Biophys Acta. 1981 Aug 27;655(1):1-8. doi: 10.1016/0005-2787(81)90059-9.
6
Structure and reactions of closed duplex DNA.闭环双链DNA的结构与反应
Annu Rev Biophys Bioeng. 1978;7:287-313. doi: 10.1146/annurev.bb.07.060178.001443.
7
The use of Haemophilus gallinarum DNA-relaxing enzyme to investigate the relationship between the number of superhelical turns and the molecular weight in a negatively twisted DNA.利用鸡嗜血杆菌DNA松弛酶研究负超螺旋DNA中超螺旋圈数与分子量之间的关系。
J Biochem. 1979 Sep;86(3):711-7. doi: 10.1093/oxfordjournals.jbchem.a132575.
8
Separation of closed circular DNA from linear DNA by electrophoresis in two dimensions in agarose gels.在琼脂糖凝胶中通过二维电泳从线性DNA中分离闭环DNA。
Nucleic Acids Res. 1981 Dec 21;9(24):6805-12. doi: 10.1093/nar/9.24.6805.
9
Supercoiled DNA.超螺旋DNA
Sci Am. 1980 Jul;243(1):100-13.
10
The androgenic regulation of superhelical-DNA nicking-closing enzyme in rat ventral prostate.大鼠腹侧前列腺中超螺旋DNA切口-封闭酶的雄激素调节作用
Biochem J. 1981 Apr 15;196(1):195-202. doi: 10.1042/bj1960195.

引用本文的文献

1
Rapid fluorometric quantification of bacterial cells using Redsafe nucleic acid stain.使用Redsafe核酸染料对细菌细胞进行快速荧光定量分析。
Iran J Microbiol. 2015 Dec;7(6):319-23.
2
Escherichia coli endonuclease VIII: cloning, sequencing, and overexpression of the nei structural gene and characterization of nei and nei nth mutants.大肠杆菌核酸内切酶VIII:nei结构基因的克隆、测序、过表达以及nei和nei nth突变体的特性分析
J Bacteriol. 1997 Jun;179(11):3773-82. doi: 10.1128/jb.179.11.3773-3782.1997.
3
Fluorometric quantitation of broth-cultured mycoplasmas by using alkaline ethidium bromide.
使用碱性溴化乙锭对肉汤培养的支原体进行荧光定量分析。
J Clin Microbiol. 1993 May;31(5):1303-7. doi: 10.1128/jcm.31.5.1303-1307.1993.
4
Repair-defective mutants of Alteromonas espejiana, the host for bacteriophage PM2.埃氏交替单胞菌(噬菌体PM2的宿主)的修复缺陷型突变体。
J Bacteriol. 1984 Feb;157(2):465-74. doi: 10.1128/jb.157.2.465-474.1984.
5
Left-handed Z-DNA regions are present in negatively supercoiled bacteriophage PM2 DNA.左手螺旋Z-DNA区域存在于负超螺旋噬菌体PM2 DNA中。
EMBO J. 1983;2(12):2123-8. doi: 10.1002/j.1460-2075.1983.tb01712.x.
6
Mung bean nuclease cleavage of a dA + dT-rich sequence or an inverted repeat sequence in supercoiled PM2 DNA depends on ionic environment.绿豆核酸酶对超螺旋PM2 DNA中富含dA + dT的序列或反向重复序列的切割取决于离子环境。
Nucleic Acids Res. 1984 Sep 25;12(18):7087-104. doi: 10.1093/nar/12.18.7087.
7
Changes in site specificity of single-strand-specific endonucleases on supercoiled PM2 DNA with temperature and ionic environment.
Nucleic Acids Res. 1984 Sep 25;12(18):7071-86. doi: 10.1093/nar/12.18.7071.
8
Exonuclease III recognizes urea residues in oxidized DNA.核酸外切酶III识别氧化DNA中的尿素残基。
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8354-8. doi: 10.1073/pnas.82.24.8354.
9
Review: ethidium fluorescence assays. Part 1. Physicochemical studies.综述:溴化乙锭荧光分析法。第1部分。物理化学研究。
Nucleic Acids Res. 1979 Oct 10;7(3):547-69. doi: 10.1093/nar/7.3.547.