综述：溴化乙锭荧光分析法。第1部分。物理化学研究。
Review: ethidium fluorescence assays. Part 1. Physicochemical studies.
作者信息
Morgan A R, Lee J S, Pulleyblank D E, Murray N L, Evans D H
出版信息
Nucleic Acids Res. 1979 Oct 10;7(3):547-69. doi: 10.1093/nar/7.3.547.
Abstract
DNA and RNA can be assayed rapidly and very sensitively by exploiting the enhanced fluorescence of ethidium intercalated into duplex regions. By assaying at different pHs and introducing a heating/cooling cycle, a great many physicochemical aspects of DNA and RNA can be studied avoiding the use of radiolabels, and often giving information not otherwise readily obtainable. Studies are described on duplex DNA which involve measurement of extinction coefficients, cross-linking by chemicals, Cot curve analysis as well as estimation of drug-DNA binding constants. The assays can be adapted to investigate multi-stranded nucleic acid structures. The use of covalently closed circular DNA also allows rapid and extremely sensitive measurements of nicking caused by irradiation or drugs.
摘要
通过利用嵌入双链区域的溴化乙锭增强的荧光，可以快速且非常灵敏地检测DNA和RNA。通过在不同pH值下进行检测并引入加热/冷却循环，可以研究DNA和RNA的许多物理化学方面，避免使用放射性标记，并且常常能提供其他方式难以获得的信息。文中描述了对双链DNA的研究，包括消光系数的测量、化学交联、Cot曲线分析以及药物与DNA结合常数的估计。这些检测方法可用于研究多链核酸结构。使用共价闭合环状DNA还可以快速且极其灵敏地测量由辐射或药物引起的切口。
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