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Changes in site specificity of single-strand-specific endonucleases on supercoiled PM2 DNA with temperature and ionic environment.

作者信息

Kowalski D

出版信息

Nucleic Acids Res. 1984 Sep 25;12(18):7071-86. doi: 10.1093/nar/12.18.7071.

DOI:10.1093/nar/12.18.7071
PMID:6091053
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320143/
Abstract

Mung bean nuclease sites in supercoiled PM2 DNA at neutral pH were located by linearizing the singly-nicked circular DNA product with venom phosphodiesterase followed by restriction endonuclease mapping. The locations of the sites varied with small changes in temperature and in concentration of NaC1 or magnesium ion. Different environmental changes which affect duplex stability in the same direction showed similar effects on the number of sites and in some cases resulted in identical cleavage patterns. Venom phosphodiesterase and P1 nuclease showed cleavage patterns similar to mung bean nuclease under the same environmental conditions and showed similar variations in cleavage patterns when environmental conditions were changed. Relaxed, closed-circular DNA was slowly cleaved at numerous sites whose locations did not vary with environment. Changes in site specificity are likely the result of environmental effects on the conformation of supercoiled DNA as opposed to effects on the single-strand-specific endonucleases themselves.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8db1/320143/8f6565b957c1/nar00336-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8db1/320143/badf74eb0670/nar00336-0160-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8db1/320143/2e605b9cfd8f/nar00336-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8db1/320143/88dc44c1010d/nar00336-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8db1/320143/209dd2da8aa2/nar00336-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8db1/320143/8f6565b957c1/nar00336-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8db1/320143/badf74eb0670/nar00336-0160-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8db1/320143/2e605b9cfd8f/nar00336-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8db1/320143/88dc44c1010d/nar00336-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8db1/320143/209dd2da8aa2/nar00336-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8db1/320143/8f6565b957c1/nar00336-0165-a.jpg

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[Kinetic parameters of superhelical DNA cleavage by endonuclease S1].
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本文引用的文献

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An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin.通过S1核酸酶检测到的DNA构象改变发生在活跃的鸡珠蛋白染色质的特定区域。
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Left-handed Z-DNA is induced by supercoiling in physiological ionic conditions.在生理离子条件下，超螺旋可诱导左手Z-DNA的形成。
Nature. 1982 Sep 23;299(5881):312-6. doi: 10.1038/299312a0.
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Action of mung bean nuclease on supercoiled PM2 DNA.绿豆核酸酶对超螺旋PM2 DNA的作用。
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