Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA.
Sensors (Basel). 2011;11(1):886-904. doi: 10.3390/s110100886. Epub 2011 Jan 14.
The development of porphyrin-embedded mesoporous organosilicate materials for application to the detection of volatile hydrocarbon solvents is described. Design of the receptor and optical indicator construct begins with parallel selection of the porphyrin indicator and design of the mesoporous sorbent. For the porphyrin indicator, high binding affinity and strong changes in spectrophotometric character upon target interaction are desired. The sorbent should provide high target binding capacity and rapid binding kinetics. A number of porphyrin/metalloporphyrin variants and organosilicate sorbents were evaluated to determine the characteristics of their interaction with the targets, benzene, toluene, and hexane. The selected porphyrin candidates were covalently immobilized within a benzene-bridged sorbent. This construct was applied to the detection of targets using both fluorescence- and reflectance-based protocols. The use of red, green, and blue (RGB) color values from the constructs in a highly simplified detection scheme is described.
本文描述了用于检测挥发性碳氢溶剂的卟啉嵌入介孔有机硅材料的开发。受体和光学指示剂结构的设计始于卟啉指示剂和介孔吸附剂的并行选择。对于卟啉指示剂，希望具有高结合亲和力和在目标相互作用时分光光度特性的强烈变化。吸附剂应提供高的目标结合容量和快速的结合动力学。评估了许多卟啉/金属卟啉变体和有机硅吸附剂，以确定它们与苯、甲苯和己烷等目标物相互作用的特性。选择的卟啉候选物被共价固定在苯桥接的吸附剂中。该结构通过荧光和反射率两种协议被应用于目标物的检测。描述了在高度简化的检测方案中使用构建体的红、绿和蓝（RGB）颜色值。
