Binning Jennifer M, Leung Daisy W, Amarasinghe Gaya K
Department of Pathology and Immunology, Washington University School of Medicine St. Louis, MO, USA.
Front Microbiol. 2012 Feb 8;3:29. doi: 10.3389/fmicb.2012.00029. eCollection 2012.
Aptamers generated from randomized libraries of nucleic acids have found utility in a wide variety of fields and in the clinic. Aptamers can be used to target both intracellular and extracellular components, including small molecules, proteins, cells, and viruses. With recent technological developments in stringent selection and rapid isolation strategies, it is likely that aptamers will continue to make an impact as useful tools and reagents. Although many recently developed aptamers are intended for use as therapeutic and diagnostic agents, use of aptamers for basic research, including target validation, remains an active area with high potential to impact our understanding of molecular mechanisms and for drug discovery. In this brief review, we will discuss recent aptamer discoveries, their potential role in structural virology, as well as challenges and future prospects.
从随机核酸文库中产生的适配体已在广泛的领域和临床中得到应用。适配体可用于靶向细胞内和细胞外成分，包括小分子、蛋白质、细胞和病毒。随着严格筛选和快速分离策略的最新技术发展，适配体很可能会继续作为有用的工具和试剂发挥作用。尽管最近开发的许多适配体旨在用作治疗和诊断试剂，但将适配体用于基础研究，包括靶点验证，仍然是一个活跃的领域，极有可能影响我们对分子机制的理解以及药物发现。在这篇简短的综述中，我们将讨论最近的适配体发现、它们在结构病毒学中的潜在作用，以及挑战和未来前景。
