利用分子方法对伊朗阴道毛滴虫ITS1基因的研究
Study on ITS1 Gene of Iranian Trichomonas vaginalis by Molecular Methods.
作者信息
Kazemi F, Hooshyar H, Zareikar B, Bandehpour M, Arbabi M, Talari S, Alizadeh R, Kazemi B
机构信息
Parasitology Dept., School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
出版信息
Iran J Parasitol. 2010 Dec;5(4):9-14.
BACKGROUND
Trichomoniasis is a worldwide protozoan parasitic disease and metronidazole is a choice drug for its treatment. Because of disease importance in public health and its controversial ideas about the prevalence of drug resistance, this study was carried out.
METHODS
Fifty-two suspected vaginal samples were collected from 2006 to 2007 in Gynecology Maryam Hospital, Tehran, Iran. All isolates were examined by microscopic, culture and PCR techniques. The PCR products were analyzed by RFLP and CSGE methods and two suspected samples were sequenced.
RESULTS
Trichomonas vaginalis was identified from all 52 samples. Of 52 isolates, 45 samples were successfully cultured and amplified by PCR except one. Seven were positive only by PCR. Finally, ITS1 fragment was successfully amplified in 51 of 52. CSGE analysis and PCR products digestion by MspI followed by sequencing showed nucleotide mutation at position 209 (C209T) of the ITS1 fragment in two (3.9%) of them.
CONCLUSION
The results showed mutation in ITS1 fragment of T. vaginalis in two (3.9%) of Iranian isolates which may be related to metronidazole resistance.
背景
滴虫病是一种全球性的原生动物寄生虫病，甲硝唑是治疗该病的首选药物。鉴于该病在公共卫生方面的重要性以及关于耐药性流行情况的争议观点，开展了本研究。
方法
2006年至2007年期间，从伊朗德黑兰玛丽安妇科医院采集了52份疑似阴道样本。所有分离株均通过显微镜检查、培养和PCR技术进行检测。PCR产物通过RFLP和CSGE方法进行分析，并对两份疑似样本进行测序。
结果
在所有52份样本中均鉴定出阴道毛滴虫。52株分离株中，除1株外，45份样本成功培养并通过PCR扩增。7份样本仅PCR呈阳性。最终，52份样本中有51份成功扩增出ITS1片段。CSGE分析以及用MspI对PCR产物进行酶切后测序显示，其中两份（3.9%）样本的ITS1片段第209位核苷酸发生突变（C209T）。
结论
结果显示，伊朗分离的阴道毛滴虫中有两份（3.9%）的ITS1片段发生突变，这可能与甲硝唑耐药性有关。
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