Matini Mohammad, Rezaei Hossein, Fallah Mohammad, Maghsood Amir Hossein, Saidijam Massoud, Shamsi-Ehsan Tayebeh
Dept. of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Dept. of Molecular Medicine and Genetics, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Iran J Parasitol. 2017 Jan-Mar;12(1):29-37.
In spite of sufficient knowledge about phenotypic variation of , its genetic characteristics are poorly understood. We carried out a molecular epidemiology study in which in vitro metronidazole susceptibility of isolates was considered.
This study was conducted on 862 women admitted to Gynecology Clinics in Hamadan, west of Iran, during 2014-2015. After recording the socio-demographic and clinical characteristics of participants, vaginal swab samples were taken and subjected to microscopic examination, culture, in vitro sensitivity testing and PCR-restriction fragment length polymorphism (RFLP) analysis.
was detected in 1.9% (16/862) of the samples using two parasitological methods. The all isolates that subjected to drug susceptibility analysis were sensitive to metronidazole with MICs ranged from 0.4 to 12.8 μg/ml. genotyping by using actin gene and PCR-RFLP analysis identified three actin type; A (9, 56%), I (6, 38%) and E (1, 6%). No significant correlation was observed between actin genotypes and their clinical manifestation (>0.05).
The prevalence of infection is not noticeable in the region and the most of isolates are hypersensitive to metronidazole. Further studies are needed to clarify the efficiency of the actin gene, as a reliable genetic marker, for molecular epidemiology of trichomoniasis.
尽管对[某种病原体名称]的表型变异有足够的了解，但其遗传特征却知之甚少。我们开展了一项分子流行病学研究，其中考虑了[某种病原体名称]分离株的体外甲硝唑敏感性。
本研究对2014 - 2015年期间入住伊朗西部哈马丹妇科诊所的862名女性进行。记录参与者的社会人口统计学和临床特征后，采集阴道拭子样本并进行显微镜检查、培养、体外敏感性测试和聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）分析。
使用两种寄生虫学方法在1.9%（16/862）的样本中检测到[某种病原体名称]。所有接受药敏分析的[某种病原体名称]分离株对甲硝唑敏感，MIC范围为0.4至12.8μg/ml。通过肌动蛋白基因和PCR - RFLP分析进行的[某种病原体名称]基因分型鉴定出三种肌动蛋白类型；A（9株，56%）、I（6株，38%）和E（1株，6%）。肌动蛋白基因型与其临床表现之间未观察到显著相关性（>0.05）。
该地区[某种病原体名称]感染的患病率不显著，且大多数分离株对甲硝唑高度敏感。需要进一步研究以阐明肌动蛋白基因作为可靠遗传标记在滴虫病分子流行病学中的作用。
