Orujzadeh Fariba, Tabatabaie Fatemeh, Khanaliha Khadijeh, Akhlaghi Lame, Bokharaei-Salim Farah, Fallah Soudabeh, Esteghamati Abdoulreza, Masoumi-Asl Hossein
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2019 Apr-Jun;14(2):250-257.
is a prevalent sexually transmitted infection cause trichomoniasis. In this study prevalence and genotype of Iranian isolates of infected (dsRNA) viruses were evaluated by PCR-RFLP and obtained patterns were then confirmed by sequence analysis and genotype of these Iranian isolates confirmed again.
Ten strains of were collected from 1700 vaginal samples of women referred to hospitals associated with Iran University of Medical Sciences in Tehran, Iran during Feb 2016 to Jul 2017, evaluated in points of infection to Virus (TVV-1) were used in a PCR-RFLP. All of ten isolates of T. vaginalis were examined by designed nested PCR for actin gene and then digestion patterns of three endonuclease enzymes of HindII, MseI and RsaI were evaluated and genotype of these isolates was defined.
By combination of fragments pattern of three enzymes of HindII, RsaI and MseI, three genotypes were found; six genotypes E, two genotypes G and two genotypes I. The most dominant genotypes were genotype E. Among four TVV infected isolates two genotype E, one genotype G and one genotype I were found, however among six uninfected isolates to TVV-1, all of three genotypes were also found.
Three genotypes E, G and I in infected with dsRNA isolates were found, however, these three genotypes in without virus were also observed. Further study is needed to evaluate genotypes of , which infected virus in more great T. vaginalis population.
是一种引起滴虫病的常见性传播感染。在本研究中，通过PCR-RFLP评估伊朗感染（dsRNA）病毒分离株的流行率和基因型，然后通过序列分析确认获得的模式，并再次确认这些伊朗分离株的基因型。
2016年2月至2017年7月期间，从转诊至伊朗德黑兰医科大学相关医院的1700名女性阴道样本中收集了10株，在感染病毒（TVV-1）的感染点进行评估，并用于PCR-RFLP。对所有10株阴道毛滴虫分离株进行肌动蛋白基因的巢式PCR检测，然后评估HindII、MseI和RsaI三种内切酶的酶切模式，并确定这些分离株的基因型。
通过HindII、RsaI和MseI三种酶的片段模式组合，发现了三种基因型；六种基因型E、两种基因型G和两种基因型I。最主要的基因型是基因型E。在4株感染TVV的分离株中，发现了两种基因型E、一种基因型G和一种基因型I，然而在6株未感染TVV-1的分离株中，也发现了所有三种基因型。
在感染dsRNA分离株的中发现了三种基因型E、G和I，然而，在未感染病毒的中也观察到了这三种基因型。需要进一步研究以评估在更大的阴道毛滴虫群体中感染病毒的的基因型。
