Tuzun Egemen, Bick Roger, Kadipasaoglu Cihan, Conger Jeffrey L, Poindexter Brian J, Gregoric Igor D, Frazier O H, Towbin Jeffrey A, Radovancevic Branislav
Cardiovascular Surgical Research Laboratories, Texas Heart Institute, Houston, TX 77225, USA.
ISRN Cardiol. 2011;2011:831062. doi: 10.5402/2011/831062. Epub 2011 Jul 6.
Purpose. To provide an ovine model of ventricular remodeling and reverse remodeling by creating congestive heart failure (CHF) and then treating it by implanting a left ventricular assist device (LVAD). Methods. We induced volume-overload heart failure in 2 sheep; 20 weeks later, we implanted an LVAD and assessed recovery 11 weeks thereafter. We examined changes in histologic and hemodynamic data and levels of cellular markers of CHF. Results. After CHF induction, we found increases in LV end-diastolic pressure, LV systolic and diastolic dimensions, wall thickness, left atrial diameter, and atrial natriuretic protein (ANP) and endothelin-1 (ET-1) levels; β-adrenergic receptor (BAR) and dystrophin expression decreased markedly. Biopsies confirmed LV remodeling. After LVAD support, LV systolic and diastolic dimensions, wall thickness, and mass, and ANP and ET-1 levels decreased. Histopathologic and hemodynamic markers improved, and BAR and dystrophin expression normalized. Conclusions. We describe a successful sheep model for ventricular and reverse remodeling.
目的。通过制造充血性心力衰竭（CHF），然后植入左心室辅助装置（LVAD）进行治疗，以提供一种心室重构和逆向重构的绵羊模型。方法。我们在2只绵羊中诱导容量超负荷心力衰竭；20周后，我们植入LVAD，并在之后11周评估恢复情况。我们检查了组织学和血流动力学数据的变化以及CHF细胞标志物的水平。结果。在诱导CHF后，我们发现左心室舒张末期压力、左心室收缩和舒张尺寸、壁厚、左心房直径以及心房利钠肽（ANP）和内皮素-1（ET-1）水平升高；β-肾上腺素能受体（BAR）和肌营养不良蛋白表达明显降低。活检证实左心室重构。在LVAD支持后，左心室收缩和舒张尺寸、壁厚和质量以及ANP和ET-1水平降低。组织病理学和血流动力学标志物改善，BAR和肌营养不良蛋白表达恢复正常。结论。我们描述了一种成功的心室和逆向重构绵羊模型。
