Malejko Julita, Szygałowicz Marzena, Godlewska-Żyłkiewicz Beata, Kojło Anatol
Institute of Chemistry, University of Bialystok, Hurtowa 1, 15-399 Białystok, Poland.
Mikrochim Acta. 2012 Feb;176(3-4):429-435. doi: 10.1007/s00604-011-0737-6. Epub 2011 Nov 20.
Fungi of the type Aspergillus sp. were immobilized on a cellulosic resin and used as a biosorbent for the on-line preconcentration and separation of Pt(IV) ions prior to their chemiluminescent determination via flow injection analysis. Biosorption and elution conditions were optimized, and the results compared to biosorbents based on the use of Chlorella vulgaris algae and Saccharomyces cerevisiae yeast in terms of preconcentration and selective retention of Pt(IV). The immobilized fungi presented here have a high potential for use in platinum biosorption. The procedure exhibits the currently lowest limit of detection (0.02 ng mL(-1) of Pt) and very high selectivity. The procedure was applied to the determination of Pt(IV) in river water, road run-off, and wastewater samples.FigureSchematic diagram of flow injection manifold for on-line preconcentration/separation of Pt(IV) on immobilized fungi followed by its luminol-based chemiluminescent determination. The CL-FIA manifold was applied to the determination of platinum in river water, road run-off, and wastewater samples.
将曲霉属真菌固定在纤维素树脂上，并用作生物吸附剂，用于在通过流动注射分析进行化学发光测定之前对Pt(IV)离子进行在线预富集和分离。对生物吸附和洗脱条件进行了优化，并将结果与基于小球藻和酿酒酵母的生物吸附剂在Pt(IV)的预富集和选择性保留方面进行了比较。本文介绍的固定化真菌在铂生物吸附方面具有很高的应用潜力。该方法具有目前最低的检测限(0.02 ng mL(-1)的Pt)和非常高的选择性。该方法应用于河水、道路径流和废水样品中Pt(IV)的测定。图用于在固定化真菌上在线预富集/分离Pt(IV)，随后基于鲁米诺进行化学发光测定的流动注射流路示意图。该化学发光-流动注射流路应用于河水、道路径流和废水样品中铂的测定。
