Postgraduate Medical Institute, University of Hull, Hull, UK.
Int J Lab Hematol. 2012 Aug;34(4):396-402. doi: 10.1111/j.1751-553X.2012.01409.x. Epub 2012 Feb 20.
Cancers are associated with varying degrees of an increased risk of venous thromboembotic events (VTE) occurring. This increased risk is tumour driven and associated with tumour expression of tissue factor (TF) and tumour-derived microparticles (MP). In this study, cancer cell lines from phenotypically distinct tumours were assessed for cell surface TF expression and prothrombin time (PT) taken as a measure of procoagulant potential.
Breast (T47D, MCF-7), colorectal (Colo320 and LoVo), head and neck (USCC 11b, 12, 81b and SIHN-011A) and pancreatic tumour cell lines (ASPC-1 and CFPAC-1) were assessed for TF expression by flow cytometry and relative mean fluorescence determined. Procoagulant potential of the cells was then determined by PT assay.
Cell-supported coagulation was shown to be cell number dependent, defined by a logarithmic relationship that was consistent across all cell lines. Single cell PT was determined for each cell line from the slope of a logarithmically transformed data plot. A near linear relationship was observed between TF expression and single cell clotting time where a higher expression of TF results in a proportionally faster PT (P < 0.001).
This study shows that across a range of tumour sites a consistent relationship is seen between procoagulant potential and both cell number and TF cell surface expression.
癌症与静脉血栓栓塞事件（VTE）风险增加的程度有关。这种风险增加是由肿瘤驱动的，与肿瘤组织因子（TF）的表达和肿瘤衍生的微粒（MP）有关。在这项研究中，评估了来自表型不同肿瘤的癌细胞系的细胞表面 TF 表达，并将凝血酶原时间（PT）作为促凝潜能的衡量标准。
通过流式细胞术评估乳腺癌（T47D、MCF-7）、结直肠癌（Colo320 和 LoVo）、头颈部（USCC 11b、12、81b 和 SIHN-011A）和胰腺癌细胞系（ASPC-1 和 CFPAC-1）的 TF 表达，并确定相对平均荧光强度。然后通过 PT 测定法确定细胞的促凝潜能。
细胞支持的凝血与细胞数量有关，这由对数关系定义，对数关系在所有细胞系中都是一致的。从对数转换数据图的斜率确定每个细胞系的单个细胞 PT。在 TF 表达和单个细胞凝血时间之间观察到近乎线性的关系，其中 TF 的表达越高，PT 就越快（P<0.001）。
本研究表明，在一系列肿瘤部位，促凝潜能与细胞数量和 TF 细胞表面表达之间存在一致的关系。
